1gy2
From Proteopedia
(New page: 200px<br /> <applet load="1gy2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gy2, resolution 1.82Å" /> '''CRYSTAL STRUCTURE O...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1GY2 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | + | 1GY2 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]] with CU and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: CUA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GY2 OCA]]. |
==Reference== | ==Reference== | ||
Crystal structures of the Met148Leu and Ser86Asp mutants of rusticyanin from Thiobacillus ferrooxidans: insights into the structural relationship with the cupredoxins and the multi copper proteins., Kanbi LD, Antonyuk S, Hough MA, Hall JF, Dodd FE, Hasnain SS, J Mol Biol. 2002 Jul 5;320(2):263-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12079384 12079384] | Crystal structures of the Met148Leu and Ser86Asp mutants of rusticyanin from Thiobacillus ferrooxidans: insights into the structural relationship with the cupredoxins and the multi copper proteins., Kanbi LD, Antonyuk S, Hough MA, Hall JF, Dodd FE, Hasnain SS, J Mol Biol. 2002 Jul 5;320(2):263-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12079384 12079384] | ||
| + | [[Category: Acidithiobacillus ferrooxidans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Thiobacillus ferrooxidans]] | ||
[[Category: Antonyuk, S.]] | [[Category: Antonyuk, S.]] | ||
[[Category: Dodd, F.]] | [[Category: Dodd, F.]] | ||
| Line 31: | Line 31: | ||
[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:58:51 2007'' |
Revision as of 09:54, 30 October 2007
|
CRYSTAL STRUCTURE OF MET148LEU RUSTICYANIN
Overview
The crystal structures of the Met148Leu and Ser86Asp mutants of, rusticyanin are presented at 1.82 and 1.65 A resolution, respectively., Both of these structures have two molecules in the asymmetric unit, compared to the one present in the crystal form of the native protein., This provides an opportunity to investigate intramolecular electron, transfer pathways in rusticyanin. The redox potential of the Met148Leu, mutant ( approximately 800 mV) is elevated compared to that of the native, protein ( approximately 670 mV at pH 3.2) while that of the Ser86Asp, mutant ( approximately 623 mV at pH 3.2) is decreased. The effect of the, Ser86Asp mutation on the hydrogen bonding near the type 1 Cu site is, discussed and hence its role in determining acid stability is examined., The type 1 Cu site ... [(full description)]
About this Structure
1GY2 is a [Single protein] structure of sequence from [Acidithiobacillus ferrooxidans] with CU and GOL as [ligands]. Structure known Active Site: CUA. Full crystallographic information is available from [OCA].
Reference
Crystal structures of the Met148Leu and Ser86Asp mutants of rusticyanin from Thiobacillus ferrooxidans: insights into the structural relationship with the cupredoxins and the multi copper proteins., Kanbi LD, Antonyuk S, Hough MA, Hall JF, Dodd FE, Hasnain SS, J Mol Biol. 2002 Jul 5;320(2):263-75. PMID:12079384
Page seeded by OCA on Tue Oct 30 11:58:51 2007
Categories: Acidithiobacillus ferrooxidans | Single protein | Antonyuk, S. | Dodd, F. | Hall, J.F. | Hasnain, S. | Hough, M.A. | Kanbi, L.D. | CU | GOL | Electron transport | M148l | Metal-binding | Mutant | Periplasmic | Rusticyanin | S86d | Signal
