Galactosidase
From Proteopedia
(New page: Crystal Structure of Galactosidase, 3e1f {{STRUCTURE_3e1f| PDB=3e1f | SIZE=300| SCENE= |right|CAPTION=Galactosidase, 3e1f }} α-galactosidase...) |
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{{STRUCTURE_3e1f| PDB=3e1f | SIZE=300| SCENE= |right|CAPTION=Galactosidase, [[3e1f]] }} | {{STRUCTURE_3e1f| PDB=3e1f | SIZE=300| SCENE= |right|CAPTION=Galactosidase, [[3e1f]] }} | ||
| - | α- | + | α-Galactosidase (Agal) hydrolyzes the terminal α-galactosyl moiety from glycoproteins and glycolipids. β-Galactosidase hydrolyzes β-galactosides into monosaccharides. |
| - | IPTG induces Bgal activity. PETG is an inhibitor. Galcatose, lactose, ONPG are substrates. Galactopyranosyl is reaction intermediate. | + | IPTG induces Bgal activity. PETG is an inhibitor. Galcatose, lactose, ONPG are substrates. Galactopyranosyl is reaction intermediate. The images at the left and at the right correspond to one representative Galactosidase, ''i.e.'' the crystal structure of Galactosidase from ''Escherichia coli'' ([[3e1f]]). |
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| + | == 3D Structures of Galactosidase == | ||
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| + | ===α-Galactosidase=== | ||
3lrk – yAgal 1 (mutant) – yeast | 3lrk – yAgal 1 (mutant) – yeast | ||
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1uas – Agal – rice | 1uas – Agal – rice | ||
| - | + | ===α-galactosidase binary complex=== | |
3h54 - hAgal B (mutant) + polysaccharide | 3h54 - hAgal B (mutant) + polysaccharide | ||
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1t0o – HjAgal + galactose | 1t0o – HjAgal + galactose | ||
| - | + | ===β-Galactosidase=== | |
3og2 – TrBgal – Trichoderma reesei | 3og2 – TrBgal – Trichoderma reesei | ||
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1tg7 – PeBgal – Penicillium | 1tg7 – PeBgal – Penicillium | ||
| - | + | ===β-Galactosidase binary complex=== | |
1xc6 - PeBgal + galactose | 1xc6 - PeBgal + galactose | ||
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1jz6 - EcBgal (mutant) + inhibitor | 1jz6 - EcBgal (mutant) + inhibitor | ||
| - | Endo-1,4- | + | ===Endo-1,4-β-galactosidase=== |
2xom, 2xon – TmEbgal carbohydrate-binding domain + -galactotriose | 2xom, 2xon – TmEbgal carbohydrate-binding domain + -galactotriose | ||
| - | 3,6-anhydro- | + | ===3,6-anhydro-α-L-galactosidase=== |
3p2n – AAgal – Zobellia galactanivorans | 3p2n – AAgal – Zobellia galactanivorans | ||
| - | 6-phospho- | + | ===6-phospho-β-D-galactosidase=== |
2pbg, 3pbg, 4pbg, 1pbg – Pgal (mutant) – Lactococcus lacti | 2pbg, 3pbg, 4pbg, 1pbg – Pgal (mutant) – Lactococcus lacti | ||
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2v4v – gal GH59 – Clostridium cellulolyticum | 2v4v – gal GH59 – Clostridium cellulolyticum | ||
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| + | [[Category:Topic Page]] | ||
Revision as of 09:51, 21 July 2011
α-Galactosidase (Agal) hydrolyzes the terminal α-galactosyl moiety from glycoproteins and glycolipids. β-Galactosidase hydrolyzes β-galactosides into monosaccharides. IPTG induces Bgal activity. PETG is an inhibitor. Galcatose, lactose, ONPG are substrates. Galactopyranosyl is reaction intermediate. The images at the left and at the right correspond to one representative Galactosidase, i.e. the crystal structure of Galactosidase from Escherichia coli (3e1f).
Contents |
3D Structures of Galactosidase
α-Galactosidase
3lrk – yAgal 1 (mutant) – yeast 3lrl, 3lrm – yAgal 1 (mutant) + polysaccharide 3lx9, 3lxa, 3lxb, 3lxc - hAgal A (mutant) – human 3hg2, 3gxn, 1r46, 1r47 – hAgal A 1zy9 – TmAgal – Thermotoga maritima 3h53 - hAgal B (mutant) 3a24 – BtAgal – Bacterioides thetaiotamicron 3a5v – Agal I – Mortierella vinacea 3mi6 – Agal – Lactobacillus brevis 1szn – HjAgal – Hypocrea jecorina 1uas – Agal – rice
α-galactosidase binary complex
3h54 - hAgal B (mutant) + polysaccharide 3h55 - hAgal B (mutant) + galactose 3igu - hAgal B (mutant) + intermediate 3hg3, 3hg5 - hAgal A (mutant) + galactose 3gxp - hAgal A + galactose 3hg4 – hAgal A + intermediate 3gxt – hAgal A + imino sugar 1t0o – HjAgal + galactose
β-Galactosidase
3og2 – TrBgal – Trichoderma reesei 3muy, 3i3e, 3iap, 3iaq, 3dyp, 1px3 – EcBgal (mutant) – Escherichia coli 1hn1, 1dp0, 1f49, 1f4a, 1f4h, 1gho, 1bgl, 1bgm - EcBgal 3dec, 3d3a, 3bga – BtBgal 1yq2 - Bgal (mutant) – Arthrobacter 1uwi - SsBgal (mutant) - Sulfolobus solfataricus 1uwq – SsBgal 1kwk – TtBgal – Thermus thermophilus 1tg7 – PeBgal – Penicillium
β-Galactosidase binary complex
1xc6 - PeBgal + galactose 1kwk – TtBgal + galactose 3ogr – TrBgal + galactose 3ogs - TrBgal + IPTG 3ogv – TrBgal + inhibitor 2ceq, 1uwr, 1uws, 1uwt, 1uwu - SsBgal + inhibitor 3muz, 3i3d, 3dyo, 1px4, 1jyx - EcBgal (mutant) + IPTG 3mv0, 3i3b, 3czj, 1jz0, 1jz1, 1jz2, 1jz3, 1jz4, 1jz5, 1jz6 - EcBgal (mutant) + intermediate 3mv1 - EcBgal (mutant) + guanidinium 3e1f, 1jz7 - EcBgal (mutant) + galactose 1jyn, 1jyy, 1jyz, 1jz8 - EcBgal (mutant) + lactose derivative 1jyn, 1jyw - EcBgal (mutant) + substrate 1jz6 - EcBgal (mutant) + inhibitor
Endo-1,4-β-galactosidase
2xom, 2xon – TmEbgal carbohydrate-binding domain + -galactotriose
3,6-anhydro-α-L-galactosidase
3p2n – AAgal – Zobellia galactanivorans
6-phospho-β-D-galactosidase
2pbg, 3pbg, 4pbg, 1pbg – Pgal (mutant) – Lactococcus lacti 2v4v – gal GH59 – Clostridium cellulolyticum
