Galactosidase
From Proteopedia
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===α-galactosidase binary complex=== | ===α-galactosidase binary complex=== | ||
| - | 3h54 - hAgal B (mutant) + polysaccharide | + | [[3h54]] - hAgal B (mutant) + polysaccharide |
3h55 - hAgal B (mutant) + galactose | 3h55 - hAgal B (mutant) + galactose | ||
3igu - hAgal B (mutant) + intermediate | 3igu - hAgal B (mutant) + intermediate | ||
Revision as of 10:00, 21 July 2011
α-Galactosidase (Agal) hydrolyzes the terminal α-galactosyl moiety from glycoproteins and glycolipids. β-Galactosidase hydrolyzes β-galactosides into monosaccharides. IPTG induces Bgal activity. PETG is an inhibitor. Galcatose, lactose, ONPG are substrates. Galactopyranosyl is reaction intermediate. The images at the left and at the right correspond to one representative Galactosidase, i.e. the crystal structure of Galactosidase from Escherichia coli (3e1f).
Contents |
3D Structures of Galactosidase
α-Galactosidase
3lrk – yAgal 1 (mutant) – yeast
3lrl, 3lrm – yAgal 1 (mutant) + polysaccharide
3lx9, 3lxa, 3lxb, 3lxc - hAgal A (mutant) – human
3hg2, 3gxn, 1r46, 1r47 – hAgal A
1zy9 – TmAgal – Thermotoga maritima
3h53 - hAgal B (mutant)
3a24 – BtAgal – Bacterioides thetaiotamicron
3a5v – Agal I – Mortierella vinacea
3mi6 – Agal – Lactobacillus brevis
1szn – HjAgal – Hypocrea jecorina
1uas – Agal – rice
α-galactosidase binary complex
3h54 - hAgal B (mutant) + polysaccharide 3h55 - hAgal B (mutant) + galactose 3igu - hAgal B (mutant) + intermediate 3hg3, 3hg5 - hAgal A (mutant) + galactose 3gxp - hAgal A + galactose 3hg4 – hAgal A + intermediate 3gxt – hAgal A + imino sugar 1t0o – HjAgal + galactose
β-Galactosidase
3og2 – TrBgal – Trichoderma reesei 3muy, 3i3e, 3iap, 3iaq, 3dyp, 1px3 – EcBgal (mutant) – Escherichia coli 1hn1, 1dp0, 1f49, 1f4a, 1f4h, 1gho, 1bgl, 1bgm - EcBgal 3dec, 3d3a, 3bga – BtBgal 1yq2 - Bgal (mutant) – Arthrobacter 1uwi - SsBgal (mutant) - Sulfolobus solfataricus 1uwq – SsBgal 1kwk – TtBgal – Thermus thermophilus 1tg7 – PeBgal – Penicillium
β-Galactosidase binary complex
1xc6 - PeBgal + galactose 1kwk – TtBgal + galactose 3ogr – TrBgal + galactose 3ogs - TrBgal + IPTG 3ogv – TrBgal + inhibitor 2ceq, 1uwr, 1uws, 1uwt, 1uwu - SsBgal + inhibitor 3muz, 3i3d, 3dyo, 1px4, 1jyx - EcBgal (mutant) + IPTG 3mv0, 3i3b, 3czj, 1jz0, 1jz1, 1jz2, 1jz3, 1jz4, 1jz5, 1jz6 - EcBgal (mutant) + intermediate 3mv1 - EcBgal (mutant) + guanidinium 3e1f, 1jz7 - EcBgal (mutant) + galactose 1jyn, 1jyy, 1jyz, 1jz8 - EcBgal (mutant) + lactose derivative 1jyn, 1jyw - EcBgal (mutant) + substrate 1jz6 - EcBgal (mutant) + inhibitor
Endo-1,4-β-galactosidase
2xom, 2xon – TmEbgal carbohydrate-binding domain + β-galactotriose
3,6-anhydro-α-L-galactosidase
3p2n – AAgal – Zobellia galactanivorans
6-phospho-β-D-galactosidase
2pbg, 3pbg, 4pbg, 1pbg – Pgal (mutant) – Lactococcus lacti 2v4v – gal GH59 – Clostridium cellulolyticum
