Galactosidase
From Proteopedia
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===α-galactosidase binary complex=== | ===α-galactosidase binary complex=== | ||
| - | [[3h54]] - hAgal B (mutant) + polysaccharide | + | [[3h54]] - hAgal B (mutant) + polysaccharide<br /> |
| - | 3h55 - hAgal B (mutant) + galactose | + | [[3h55]] - hAgal B (mutant) + galactose<br /> |
| - | 3igu - hAgal B (mutant) + intermediate | + | [[3igu]] - hAgal B (mutant) + intermediate<br /> |
| - | 3hg3, 3hg5 - hAgal A (mutant) + galactose | + | [[3hg3]], [[3hg5]] - hAgal A (mutant) + galactose<br /> |
| - | 3gxp - hAgal A + galactose | + | [[3gxp]] - hAgal A + galactose<br /> |
| - | 3hg4 – hAgal A + intermediate | + | [[3hg4]] – hAgal A + intermediate<br /> |
| - | 3gxt – hAgal A + imino sugar | + | [[3gxt]] – hAgal A + imino sugar<br /> |
| - | 1t0o – HjAgal + galactose | + | [[1t0o]] – HjAgal + galactose<br /> |
===β-Galactosidase=== | ===β-Galactosidase=== | ||
| - | 3og2 – TrBgal – Trichoderma reesei | + | [[3og2]] – TrBgal – ''Trichoderma reesei''<br /> |
| - | 3muy, 3i3e, 3iap, 3iaq, 3dyp, 1px3 – EcBgal (mutant) – Escherichia coli | + | [[3muy]], [[3i3e]], [[3iap]], [[3iaq]], [[3dyp]], [[1px3]] – EcBgal (mutant) – ''Escherichia coli''<br /> |
| - | 1hn1, 1dp0, 1f49, 1f4a, 1f4h, 1gho, 1bgl, 1bgm - EcBgal | + | [[1hn1]], [[1dp0]], [[1f49]], [[1f4a]], [[1f4h]], [[1gho]], [[1bgl]], [[1bgm]] - EcBgal<br /> |
| - | 3dec, 3d3a, 3bga – BtBgal | + | [[3dec]], [[3d3a]], [[3bga]] – BtBgal<br /> |
| - | 1yq2 - Bgal (mutant) – Arthrobacter | + | [[1yq2]] - Bgal (mutant) – Arthrobacter<br /> |
| - | 1uwi - SsBgal (mutant) - Sulfolobus solfataricus | + | [[1uwi]] - SsBgal (mutant) - ''Sulfolobus solfataricus''<br /> |
| - | 1uwq – SsBgal | + | [[1uwq]] – SsBgal<br /> |
| - | 1kwk – TtBgal – Thermus thermophilus | + | [[1kwk]] – TtBgal – ''Thermus thermophilus''<br /> |
| - | 1tg7 – PeBgal – Penicillium | + | [[1tg7]] – PeBgal – Penicillium<br /> |
===β-Galactosidase binary complex=== | ===β-Galactosidase binary complex=== | ||
Revision as of 10:04, 21 July 2011
α-Galactosidase (Agal) hydrolyzes the terminal α-galactosyl moiety from glycoproteins and glycolipids. β-Galactosidase hydrolyzes β-galactosides into monosaccharides. IPTG induces Bgal activity. PETG is an inhibitor. Galcatose, lactose, ONPG are substrates. Galactopyranosyl is reaction intermediate. The images at the left and at the right correspond to one representative Galactosidase, i.e. the crystal structure of Galactosidase from Escherichia coli (3e1f).
Contents |
3D Structures of Galactosidase
α-Galactosidase
3lrk – yAgal 1 (mutant) – yeast
3lrl, 3lrm – yAgal 1 (mutant) + polysaccharide
3lx9, 3lxa, 3lxb, 3lxc - hAgal A (mutant) – human
3hg2, 3gxn, 1r46, 1r47 – hAgal A
1zy9 – TmAgal – Thermotoga maritima
3h53 - hAgal B (mutant)
3a24 – BtAgal – Bacterioides thetaiotamicron
3a5v – Agal I – Mortierella vinacea
3mi6 – Agal – Lactobacillus brevis
1szn – HjAgal – Hypocrea jecorina
1uas – Agal – rice
α-galactosidase binary complex
3h54 - hAgal B (mutant) + polysaccharide
3h55 - hAgal B (mutant) + galactose
3igu - hAgal B (mutant) + intermediate
3hg3, 3hg5 - hAgal A (mutant) + galactose
3gxp - hAgal A + galactose
3hg4 – hAgal A + intermediate
3gxt – hAgal A + imino sugar
1t0o – HjAgal + galactose
β-Galactosidase
3og2 – TrBgal – Trichoderma reesei
3muy, 3i3e, 3iap, 3iaq, 3dyp, 1px3 – EcBgal (mutant) – Escherichia coli
1hn1, 1dp0, 1f49, 1f4a, 1f4h, 1gho, 1bgl, 1bgm - EcBgal
3dec, 3d3a, 3bga – BtBgal
1yq2 - Bgal (mutant) – Arthrobacter
1uwi - SsBgal (mutant) - Sulfolobus solfataricus
1uwq – SsBgal
1kwk – TtBgal – Thermus thermophilus
1tg7 – PeBgal – Penicillium
β-Galactosidase binary complex
1xc6 - PeBgal + galactose 1kwk – TtBgal + galactose 3ogr – TrBgal + galactose 3ogs - TrBgal + IPTG 3ogv – TrBgal + inhibitor 2ceq, 1uwr, 1uws, 1uwt, 1uwu - SsBgal + inhibitor 3muz, 3i3d, 3dyo, 1px4, 1jyx - EcBgal (mutant) + IPTG 3mv0, 3i3b, 3czj, 1jz0, 1jz1, 1jz2, 1jz3, 1jz4, 1jz5, 1jz6 - EcBgal (mutant) + intermediate 3mv1 - EcBgal (mutant) + guanidinium 3e1f, 1jz7 - EcBgal (mutant) + galactose 1jyn, 1jyy, 1jyz, 1jz8 - EcBgal (mutant) + lactose derivative 1jyn, 1jyw - EcBgal (mutant) + substrate 1jz6 - EcBgal (mutant) + inhibitor
Endo-1,4-β-galactosidase
2xom, 2xon – TmEbgal carbohydrate-binding domain + β-galactotriose
3,6-anhydro-α-L-galactosidase
3p2n – AAgal – Zobellia galactanivorans
6-phospho-β-D-galactosidase
2pbg, 3pbg, 4pbg, 1pbg – Pgal (mutant) – Lactococcus lacti 2v4v – gal GH59 – Clostridium cellulolyticum
