2cli
From Proteopedia
(New page: 200px<br /> <applet load="2cli" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cli, resolution 1.70Å" /> '''TRYPTOPHAN SYNTHASE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2CLI is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from | + | 2CLI is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with NA, F9F and PLP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
| + | [[Category: Tryptophan synthase]] | ||
[[Category: Barends, T.R.]] | [[Category: Barends, T.R.]] | ||
[[Category: Blumenstein, L.]] | [[Category: Blumenstein, L.]] | ||
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[[Category: tryptophan biosynthesis]] | [[Category: tryptophan biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:18:14 2007'' |
Revision as of 10:13, 30 October 2007
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TRYPTOPHAN SYNTHASE IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZENESULFONYL)-2-AMINO-1-ETHYLPHOSPHATE (F9)
Overview
Allosteric interactions regulate substrate channeling in Salmonella, typhimurium tryptophan synthase. The channeling of indole between the, alpha- and beta-sites via the interconnecting 25 A tunnel is regulated by, allosteric signaling arising from binding of ligand to the alpha-site, and, covalent reaction of l-Ser at the beta-site. This signaling switches the, alpha- and beta-subunits between open conformations of low activity and, closed conformations of high activity. Our objective is to synthesize and, characterize new classes of alpha-site ligands (ASLs) that mimic the, binding of substrates, 3-indole-d-glycerol 3'-phosphate (IGP) or, d-glyceraldehyde 3-phosphate (G3P), for use in the investigation of, alpha-site-beta-site interactions. The new synthesized IGP analogues, contain an ... [(full description)]
About this Structure
2CLI is a [Protein complex] structure of sequences from [Salmonella typhimurium] with NA, F9F and PLP as [ligands]. Active as [Tryptophan synthase], with EC number [4.2.1.20]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex., Ngo H, Harris R, Kimmich N, Casino P, Niks D, Blumenstein L, Barends TR, Kulik V, Weyand M, Schlichting I, Dunn MF, Biochemistry. 2007 Jul 3;46(26):7713-27. Epub 2007 Jun 9. PMID:17559195
Page seeded by OCA on Tue Oct 30 12:18:14 2007
Categories: Protein complex | Salmonella typhimurium | Tryptophan synthase | Barends, T.R. | Blumenstein, L. | Casino, P. | Dunn, M.F. | Harris, R. | Kimmich, N. | Kulik, V. | Ngo, H. | Niks, D. | Schlichting, I. | Weyand, M. | F9F | NA | PLP | Allosteric enzyme | Amino-acid biosynthesis | Aromatic amino acid biosynthesis | Carbon- oxygen lyase | Lyase | Pyridoxal phosphate | Tryptophan biosynthesis
