5bca
From Proteopedia
(New page: 200px<br /> <applet load="5bca" size="450" color="white" frame="true" align="right" spinBox="true" caption="5bca, resolution 2.2Å" /> '''BETA-AMYLASE FROM BA...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 5BCA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5BCA OCA]]. | + | 5BCA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Beta-amylase Beta-amylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2]]. Structure known Active Sites: CAA, CAB, CAC and CAD. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5BCA OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of beta-amylase from Bacillus cereus var. mycoides at 2.2 A resolution., Oyama T, Kusunoki M, Kishimoto Y, Takasaki Y, Nitta Y, J Biochem (Tokyo). 1999 Jun;125(6):1120-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10348915 10348915] | Crystal structure of beta-amylase from Bacillus cereus var. mycoides at 2.2 A resolution., Oyama T, Kusunoki M, Kishimoto Y, Takasaki Y, Nitta Y, J Biochem (Tokyo). 1999 Jun;125(6):1120-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10348915 10348915] | ||
[[Category: Bacillus cereus]] | [[Category: Bacillus cereus]] | ||
| + | [[Category: Beta-amylase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Kishimoto, Y.]] | [[Category: Kishimoto, Y.]] | ||
| Line 24: | Line 25: | ||
[[Category: raw-starch binding domain]] | [[Category: raw-starch binding domain]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:20:00 2007'' |
Revision as of 10:15, 30 October 2007
|
BETA-AMYLASE FROM BACILLUS CEREUS VAR. MYCOIDES
Overview
The crystal structure of beta-amylase from Bacillus cereus var. mycoides, was determined by the multiple isomorphous replacement method. The, structure was refined to a final R-factor of 0.186 for 102,807 independent, reflections with F/sigma(F) > or = 2.0 at 2.2 A resolution with, root-mean-square deviations from ideality in bond lengths, and bond angles, of 0.014 A and 3.00 degrees, respectively. The asymmetric unit comprises, four molecules exhibiting a dimer-of-dimers structure. The enzyme, however, acts as a monomer in solution. The beta-amylase molecule folds, into three domains; the first one is the N-terminal catalytic domain with, a (beta/alpha)8 barrel, the second one is the excursion part from the, first one, and the third one is the C-terminal domain with two almost, ... [(full description)]
About this Structure
5BCA is a [Single protein] structure of sequence from [Bacillus cereus] with CA as [ligand]. Active as [Beta-amylase], with EC number [3.2.1.2]. Structure known Active Sites: CAA, CAB, CAC and CAD. Full crystallographic information is available from [OCA].
Reference
Crystal structure of beta-amylase from Bacillus cereus var. mycoides at 2.2 A resolution., Oyama T, Kusunoki M, Kishimoto Y, Takasaki Y, Nitta Y, J Biochem (Tokyo). 1999 Jun;125(6):1120-30. PMID:10348915
Page seeded by OCA on Tue Oct 30 12:20:00 2007
