1h2f
From Proteopedia
(New page: 200px<br /> <applet load="1h2f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h2f, resolution 2.00Å" /> '''BACILLUS STEAROTHER...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H2F is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | + | 1H2F is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]] with PO4 and VA3 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: VA3. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H2F OCA]]. |
==Reference== | ==Reference== | ||
Structures of phosphate and trivanadate complexes of Bacillus stearothermophilus phosphatase PhoE: structural and functional analysis in the cofactor-dependent phosphoglycerate mutase superfamily., Rigden DJ, Littlejohn JE, Henderson K, Jedrzejas MJ, J Mol Biol. 2003 Jan 17;325(3):411-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12498792 12498792] | Structures of phosphate and trivanadate complexes of Bacillus stearothermophilus phosphatase PhoE: structural and functional analysis in the cofactor-dependent phosphoglycerate mutase superfamily., Rigden DJ, Littlejohn JE, Henderson K, Jedrzejas MJ, J Mol Biol. 2003 Jan 17;325(3):411-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12498792 12498792] | ||
| - | [[Category: | + | [[Category: Geobacillus stearothermophilus]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Jedrzejas, M.J.]] | [[Category: Jedrzejas, M.J.]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:24:52 2007'' |
Revision as of 10:20, 30 October 2007
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BACILLUS STEAROTHERMOPHILUS PHOE (PREVIOUSLY KNOWN AS YHFR) IN COMPLEX WITH TRIVANADATE
Overview
Bacillus stearothermophilus phosphatase PhoE is a member of the, cofactor-dependent phosphoglycerate mutase superfamily possessing broad, specificity phosphatase activity. Its previous structural determination in, complex with glycerol revealed probable bases for its efficient hydrolysis, of both large, hydrophobic, and smaller, hydrophilic substrates. Here we, report two further structures of PhoE complexes, to higher resolution of, diffraction, which yield a better and thorough understanding of its, catalytic mechanism. The environment of the phosphate ion in the catalytic, site of the first complex strongly suggests an acid-base catalytic, function for Glu83. It also reveals how the C-terminal tail ordering is, linked to enzyme activation on phosphate binding by a different mechanism, ... [(full description)]
About this Structure
1H2F is a [Single protein] structure of sequence from [Geobacillus stearothermophilus] with PO4 and VA3 as [ligands]. Structure known Active Site: VA3. Full crystallographic information is available from [OCA].
Reference
Structures of phosphate and trivanadate complexes of Bacillus stearothermophilus phosphatase PhoE: structural and functional analysis in the cofactor-dependent phosphoglycerate mutase superfamily., Rigden DJ, Littlejohn JE, Henderson K, Jedrzejas MJ, J Mol Biol. 2003 Jan 17;325(3):411-20. PMID:12498792
Page seeded by OCA on Tue Oct 30 12:24:52 2007
