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1a65
From Proteopedia
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| - | [[Image:1a65.jpg|left|200px]]<br /><applet load="1a65" size=" | + | [[Image:1a65.jpg|left|200px]]<br /><applet load="1a65" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1a65, resolution 2.23Å" /> | caption="1a65, resolution 2.23Å" /> | ||
'''TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS'''<br /> | '''TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A65 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea] with NAG, GLC, CU and O as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] Known structural/functional Sites: <scene name='pdbsite=T1:Type 1 Cu Binding Site'>T1</scene> and <scene name='pdbsite=T3:Type 3 Cu Binding Site'>T3</scene>. Full crystallographic information is available from [http:// | + | 1A65 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=O:'>O</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] Known structural/functional Sites: <scene name='pdbsite=T1:Type+1+Cu+Binding+Site'>T1</scene> and <scene name='pdbsite=T3:Type+3+Cu+Binding+Site'>T3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A65 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: type-2 copper depleted]] | [[Category: type-2 copper depleted]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:29:17 2008'' |
Revision as of 07:29, 3 February 2008
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TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS
Overview
Laccase catalyses the oxidation of a variety of organic substrates coupled, to the reduction of oxygen to water. It is widely believed to be the, simplest representative of the ubiquitous blue multi-copper oxidase, family. Laccase is implicated in a wide spectrum of biological activities, and, in particular, plays a key role in morphogenesis, development and, lignin metabolism in fungi and plants. The structure of laccase from the, fungus Coprinus cinereus has been determined by X-ray crystallography at a, resolution of 2.2 A. Laccase is a monomer composed of three, cupredoxin-like beta-sandwich domains, similar to that found in ascorbate, oxidase. In contrast to ascorbate oxidase, however, the mononuclear type-1, Cu site lacks the axial methionine ligand and so exhibits trigonal planar, coordination, consistent with its elevated redox potential. Crucially, the, structure is trapped in a Cu depleted form in which the putative type-2 Cu, atom is completely absent, but in which the remaining type-1 and type-3 Cu, sites display full occupancy. Type-2 Cu depletion has unexpected, consequences for the coordination of the remaining type-3 Cu atoms.
About this Structure
1A65 is a Single protein structure of sequence from Coprinopsis cinerea with , , and as ligands. Active as Laccase, with EC number 1.10.3.2 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution., Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, Yaver DS, Pedersen AH, Davies GJ, Nat Struct Biol. 1998 Apr;5(4):310-6. PMID:9546223
Page seeded by OCA on Sun Feb 3 09:29:17 2008
Categories: Coprinopsis cinerea | Laccase | Single protein | Brzozowski, W. | Ducros, V. | CU | GLC | NAG | O | Blue multi-copper oxidase | Glycoprotein | Oxidoreductase | Signal | Type-2 copper depleted
