1acd
From Proteopedia
(New page: 200px<br /> <applet load="1acd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1acd, resolution 2.70Å" /> '''V32D/F57H MUTANT OF...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1ACD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ACD OCA]]. | + | 1ACD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]]. Structure known Active Site: POR. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ACD OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: fatty acid binding protein]] | [[Category: fatty acid binding protein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:26:56 2007'' |
Revision as of 10:22, 30 October 2007
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V32D/F57H MUTANT OF MURINE ADIPOCYTE LIPID BINDING PROTEIN
Overview
A number of crystallographic studies of the adipocyte lipid-binding, protein have established that the fatty acid-binding site is within an, internalized water-filled cavity. The same studies have also suggested the, existence of a region physically distinct from the fatty acid-binding site, which connects the cavity of the protein with the external solvent, hereafter referred to as the portal. In an effort to examine the portal, region, we have used site-directed mutagenesis to introduce the mutations, V32D/F57H into the murine ALBP cDNA. Mutant protein has been isolated, crystallized, and its stability and binding properties studied by, biochemical methods. As assessed by guanidine-HCl denaturation, the mutant, form exhibited a slight overall destabilization relative to the wild-type, ... [(full description)]
About this Structure
1ACD is a [Single protein] structure of sequence from [Mus musculus]. Structure known Active Site: POR. Full crystallographic information is available from [OCA].
Reference
Biochemical and crystallographic analyses of a portal mutant of the adipocyte lipid-binding protein., Ory J, Kane CD, Simpson MA, Banaszak LJ, Bernlohr DA, J Biol Chem. 1997 Apr 11;272(15):9793-801. PMID:9092513
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