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1bs9
From Proteopedia
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| - | [[Image:1bs9.jpg|left|200px]]<br /><applet load="1bs9" size=" | + | [[Image:1bs9.jpg|left|200px]]<br /><applet load="1bs9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bs9, resolution 1.10Å" /> | caption="1bs9, resolution 1.10Å" /> | ||
'''ACETYLXYLAN ESTERASE FROM P. PURPUROGENUM REFINED AT 1.10 ANGSTROMS'''<br /> | '''ACETYLXYLAN ESTERASE FROM P. PURPUROGENUM REFINED AT 1.10 ANGSTROMS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BS9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_purpurogenum Penicillium purpurogenum] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetylesterase Acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.6 3.1.1.6] Known structural/functional Site: <scene name='pdbsite=CAT:These Three Residues Form The Catalytic Triad'>CAT</scene>. Full crystallographic information is available from [http:// | + | 1BS9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_purpurogenum Penicillium purpurogenum] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetylesterase Acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.6 3.1.1.6] Known structural/functional Site: <scene name='pdbsite=CAT:These+Three+Residues+Form+The+Catalytic+Triad'>CAT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BS9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: serine hydrolase]] | [[Category: serine hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:33:48 2008'' |
Revision as of 07:33, 3 February 2008
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ACETYLXYLAN ESTERASE FROM P. PURPUROGENUM REFINED AT 1.10 ANGSTROMS
Overview
Enzymatic and non-enzymatic iodination of the amino acid tyrosine is a, well known phenomenon. The iodination technique has been widely used for, labeling proteins. Using high-resolution X-ray crystallographic, techniques, the chemical and three-dimensional structures of iodotyrosines, formed by non-enzymatic incorporation of I atoms into tyrosine residues of, a crystalline protein are described. Acetylxylan esterase (AXE II; 207, amino-acid residues) from Penicillium purpurogenum has substrate, specificities towards acetate esters of D-xylopyranose residues in xylan, and belongs to a new class of alpha/beta hydrolases. The crystals of the, enzyme are highly ordered, tightly packed and diffract to better than, sub-angstrom resolution at 85 K. The iodination technique has been, utilized to prepare an isomorphous derivative of the AXE II crystal. The, structure of the enzyme determined at 1.10 A resolution exclusively by, normal and anomalous scattering from I atoms, along with the structure of, the iodinated complex at 1.80 A resolution, demonstrate the formation of, covalent bonds between I atoms and C atoms at ortho positions to the, hydroxyl groups of two tyrosyl moieties, yielding iodotyrosines.
About this Structure
1BS9 is a Single protein structure of sequence from Penicillium purpurogenum with as ligand. Active as Acetylesterase, with EC number 3.1.1.6 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase., Ghosh D, Erman M, Sawicki M, Lala P, Weeks DR, Li N, Pangborn W, Thiel DJ, Jornvall H, Gutierrez R, Eyzaguirre J, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84. PMID:10089308
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