1gor
From Proteopedia
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| - | [[Image:1gor.gif|left|200px]]<br /><applet load="1gor" size=" | + | [[Image:1gor.gif|left|200px]]<br /><applet load="1gor" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gor, resolution 1.7Å" /> | caption="1gor, resolution 1.7Å" /> | ||
'''THERMOSTABLE XYLANASE I FROM THERMOASCUS AURANTIACUS-XYLOBIOSE COMPLEX AT 100 K'''<br /> | '''THERMOSTABLE XYLANASE I FROM THERMOASCUS AURANTIACUS-XYLOBIOSE COMPLEX AT 100 K'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GOR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Known structural/functional Site: <scene name='pdbsite=ACI:Xyp Binding Site'>ACI</scene>. Full crystallographic information is available from [http:// | + | 1GOR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Known structural/functional Site: <scene name='pdbsite=ACI:Xyp+Binding+Site'>ACI</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GOR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: xylanase]] | [[Category: xylanase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:41:26 2008'' |
Revision as of 07:41, 3 February 2008
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THERMOSTABLE XYLANASE I FROM THERMOASCUS AURANTIACUS-XYLOBIOSE COMPLEX AT 100 K
Overview
The substrate specificity of Thermoascus aurantiacus xylanase 10A (TAX), has been investigated both biochemically and structurally. High resolution, crystallographic analyses at 291 K and 100 K of TAX complexes with, xylobiose show that the ligand is in its alpha anomeric conformation and, provide a rationale for specificity on p-nitrophenyl glycosides at the -1, and -2 subsites. Trp 275, which is disordered in uncomplexed structures, is stabilised by its interaction with xylobiose. Two structural subsets in, family 10 are identified, which differ by the presence or absence of a, short helical stretch in the eighth betaalpha-loop of the TIM barrel, the, loop bearing Trp 275. This structural difference is discussed in the, context of Trp 275 mobility and xylanase function.
About this Structure
1GOR is a Single protein structure of sequence from Thermoascus aurantiacus. Active as Endo-1,4-beta-xylanase, with EC number 3.2.1.8 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Substrate specificity and subsite mobility in T. aurantiacus xylanase 10A., Lo Leggio L, Kalogiannis S, Eckert K, Teixeira SC, Bhat MK, Andrei C, Pickersgill RW, Larsen S, FEBS Lett. 2001 Dec 7;509(2):303-8. PMID:11741607
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