1h1x

From Proteopedia

Revision as of 07:45, 3 February 2008

SPERM WHALE MYOGLOBIN MUTANT T67R S92D

Overview

Atomic co-ordinates and structure factors for the T67R/S92D metMbCN mutant, have been deposited with the Protein Data Bank, under accession codes 1h1x, and r1h1xsf, respectively. Protein engineering and cofactor replacement, have been employed as tools to introduce/modulate peroxidase activity in, sperm whale Mb (myoglobin). Based on the rationale that haem peroxidase, active sites are characterized by specific charged residues, the Mb haem, crevice has been modified to host a haem-distalpropionate Arg residue and, a proximal Asp, yielding the T67R/S92D Mb mutant. To code extra, conformational mobility around the haem, and to increase the peroxidase, catalytic efficiency, the T67R/S92D Mb mutant has been subsequently, reconstituted with protohaem-L-histidine methyl ester, yielding a stable, derivative, T67R/S92D Mb-H. The crystal structure of T67R/S92D cyano-metMb, (1.4 A resolution; R factor, 0.12) highlights a regular haem-cyanide, binding mode, and the role for the mutated residues in affecting the haem, propionates as well as the neighbouring water structure. The, conformational disorder of the haem propionate-7 is evidenced by the NMR, spectrum of the mutant. Ligand-binding studies show that the iron(III), centres of T67R/S92D Mb, and especially of T67R/S92D Mb-H, exhibit higher, affinity for azide and imidazole than wild-type Mb. In addition, both, protein derivatives react faster than wild-type Mb with hydrogen peroxide, showing higher peroxidase-like activity towards phenolic substrates. The, catalytic efficiency of T67R/S92D Mb-H in these reactions is the highest, so far reported for Mb derivatives. A model for the protein-substrate, interaction is deduced based on the crystal structure and on the NMR, spectra of protein-phenol complexes.

About this Structure

1H1X is a Single protein structure of sequence from Physeter catodon with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Engineering peroxidase activity in myoglobin: the haem cavity structure and peroxide activation in the T67R/S92D mutant and its derivative reconstituted with protohaemin-l-histidine., Roncone R, Monzani E, Murtas M, Battaini G, Pennati A, Sanangelantoni AM, Zuccotti S, Bolognesi M, Casella L, Biochem J. 2004 Feb 1;377(Pt 3):717-24. PMID:14563209

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