1h5v

From Proteopedia

Revision as of 07:46, 3 February 2008

THIOPENTASACCHARIDE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHARENS AT 1.1 A RESOLUTION IN THE TETRAGONAL CRYSTAL FORM

Overview

Many three-dimensional structures of retaining beta-D-glycoside hydrolases, have been determined, yet oligosaccharide complexes in which the ligand, spans the catalytic centre are rare. Those that have been reported so far, have revealed two modes of binding: those in which the substrate adopts a, distorted skew-boat or envelope conformation in the -1 subsite, reflecting, the distortion observed during the catalytic cycle, and those which bypass, the true catalytic centre and thus lie in a non-productive manner across, the -1 subsite. The three-dimensional structure of a retaining, endocellulase, Bacillus agaradhaerens Cel5A, in complex with methyl, 4,4(II),4(III),4(IV)-tetrathio-alpha-cellopentoside falls into this latter, category. The 1.1 A structure reveals the binding of five pyranosides, all, in the (4)C(1) chair conformation, occupying the -3, -2, +1 and +2, subsites whilst evading the catalytic machinery located in the true -1, subsite. Such binding is in marked contrast to the structure of another, retaining endocellulase, the Fusarium oxysporum Cel7B, the identical, ligand in which displayed a distorted skew-boat conformation at the active, centre. These two binding modes may reflect different steps in the binding, and catalytic process.

About this Structure

1H5V is a Single protein structure of sequence from Bacillus agaradhaerens with , , , , and as ligands. Active as Cellulase, with EC number 3.2.1.4 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Atomic resolution structure of endoglucanase Cel5A in complex with methyl 4,4II,4III,4IV-tetrathio-alpha-cellopentoside highlights the alternative binding modes targeted by substrate mimics., Varrot A, Schulein M, Fruchard S, Driguez H, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1739-42. Epub 2001, Oct 25. PMID:11679762

Page seeded by OCA on Sun Feb 3 09:46:50 2008