This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1lxt
From Proteopedia
Revision as of 07:53, 3 February 2008
|
STRUCTURE OF PHOSPHOTRANSFERASE PHOSPHOGLUCOMUTASE FROM RABBIT
Overview
Data between 6.0 and 2.4 A resolution, collected at 253 K, wer used to, refine a revised atomic model of muscle phosphoglucomutase: final, crystallographic R factor = 16.3% (Rfree = 19.1%); final r.m.s. deviations, from ideal bond lengths and angles = 0.018 A and 3.2 degrees, respectively. Features of the protein that were recognized only in the, revised model include: the disposition of water molecules within, domain-domain interfaces; two ion pairs buried in domain-domain, interfaces, one of which is a structural arginine around which the, active-site phosphoserine loop is wound; the basic architecture of the, active-site 'crevice', which is a groove in a 1(1/3)-turn helix, open at, both ends, that is produced by the interfacing of the four domains; the, distorted hexacoordinate ligand sphere of the active-site Mg2+, where the, enzymic phosphate group acts as a bidentate ligand; a pair of arginine, residues in domain IV that form part of the enzymic phosphate-binding site, (distal subsite) whose disposition in the two monomers of the asymmetric, unit is affected unequally by distant crystallographic contacts;, structural differences throughout domain IV, produced by these differing, contacts, that may mimic solution differences induced by substrate, binding; large differences in individually refined Debye-Waller thermal, factors for corresponding main-chain atoms in monomers (1) and (2), suggesting a dynamic disorder within the crystal that may involve, domain-size groups of residues; and a 'nucleophilic elbow' in the active, site that resides in a topological environment differing from previous, descriptions of this type of structure in other proteins.
About this Structure
1LXT is a Single protein structure of sequence from Oryctolagus cuniculus with and as ligands. Active as Phosphoglucomutase, with EC number 5.4.2.2 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Structure of rabbit muscle phosphoglucomutase refined at 2.4 A resolution., Liu Y, Ray WJ Jr, Baranidharan S, Acta Crystallogr D Biol Crystallogr. 1997 Jul 1;53(Pt 4):392-405. PMID:15299905
Page seeded by OCA on Sun Feb 3 09:53:26 2008
