1o76
From Proteopedia
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| - | [[Image:1o76.jpg|left|200px]]<br /><applet load="1o76" size=" | + | [[Image:1o76.jpg|left|200px]]<br /><applet load="1o76" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1o76, resolution 1.8Å" /> | caption="1o76, resolution 1.8Å" /> | ||
'''CYANIDE COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA'''<br /> | '''CYANIDE COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1O76 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with CYN, K, HEM, CAM and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1] Known structural/functional Site: <scene name='pdbsite=AC1:K Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1O76 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=CYN:'>CYN</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=CAM:'>CAM</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1] Known structural/functional Site: <scene name='pdbsite=AC1:K+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O76 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:54:21 2008'' |
Revision as of 07:54, 3 February 2008
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CYANIDE COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA
Overview
The crystal structure of the ternary cyanide complex of P450cam and, camphor was determined to 1.8A resolution and found to be identical with, the structure of the active oxygen complex [I. Schlichting et al., 2000, Science 287, 1615]. Notably, cyanide binds in a bent mode and induces the, active conformation that is characterized by the presence of two water, molecules and a flip of the carbonyl of the conserved Asp251. The, structure of the ternary complex of cyanide, L-arginine, and the oxygenase, domain of inducible nitric oxide synthase was determined to 2.4A, resolution. Cyanide binds essentially linearly, interacts with L-Arg, and, induces the binding of a water molecule at the active site. This water is, positioned by backbone interactions, located 2.8A from the nitrogen atom, of cyanide, and could provide a proton required for O-O bond scission in, the hydroxylation reaction of nitric oxide synthase.
About this Structure
1O76 is a Single protein structure of sequence from Pseudomonas putida with , , , and as ligands. Active as Camphor 5-monooxygenase, with EC number 1.14.15.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structures of cyanide complexes of P450cam and the oxygenase domain of inducible nitric oxide synthase-structural models of the short-lived oxygen complexes., Fedorov R, Ghosh DK, Schlichting I, Arch Biochem Biophys. 2003 Jan 1;409(1):25-31. PMID:12464241
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Categories: Camphor 5-monooxygenase | Pseudomonas putida | Single protein | Fedorov, R. | Ghosh, D. | Schlichting, I. | CAM | CYN | HEM | K | TRS | Electron transport | Heme | Mono-oxygenase | Oxidoreductase
