1oau
From Proteopedia
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| - | [[Image:1oau.jpg|left|200px]]<br /><applet load="1oau" size=" | + | [[Image:1oau.jpg|left|200px]]<br /><applet load="1oau" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1oau, resolution 1.8Å" /> | caption="1oau, resolution 1.8Å" /> | ||
'''FV STRUCTURE OF THE IGE SPE-7 IN COMPLEX WITH DNP-SER (IMMUNISING HAPTEN)'''<br /> | '''FV STRUCTURE OF THE IGE SPE-7 IN COMPLEX WITH DNP-SER (IMMUNISING HAPTEN)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OAU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with SER and IMD as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Imd Binding Site For Chain N'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1OAU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with <scene name='pdbligand=SER:'>SER</scene> and <scene name='pdbligand=IMD:'>IMD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Imd+Binding+Site+For+Chain+N'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: multispecificity]] | [[Category: multispecificity]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:55:41 2008'' |
Revision as of 07:55, 3 February 2008
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FV STRUCTURE OF THE IGE SPE-7 IN COMPLEX WITH DNP-SER (IMMUNISING HAPTEN)
Overview
A single antibody was shown to adopt different binding-site conformations, and thereby bind unrelated antigens. Analysis by both x-ray, crystallography and pre-steady-state kinetics revealed an equilibrium, between different preexisting isomers, one of which possessed a, promiscuous, low-affinity binding site for aromatic ligands, including the, immunizing hapten. A subsequent induced-fit isomerization led to, high-affinity complexes with a deep and narrow binding site. A protein, antigen identified by repertoire selection made use of an unrelated, antibody isomer with a wide, shallow binding site. Conformational, diversity, whereby one sequence adopts multiple structures and multiple, functions, can increase the effective size of the antibody repertoire but, may also lead to autoimmunity and allergy.
About this Structure
1OAU is a Single protein structure of sequence from Rattus rattus with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:12610298
Page seeded by OCA on Sun Feb 3 09:55:41 2008
Categories: Rattus rattus | Single protein | James, L.C. | Roversi, P. | Tawfik, D. | IMD | SER | Allergy | Antibody | Conformational diversity | Ige | Multispecificity
