This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1v1q
From Proteopedia
(New page: 200px<br /> <applet load="1v1q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v1q, resolution 2.10Å" /> '''CRYSTAL STRUCTURE O...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1V1Q is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with CYS and CYS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V1Q OCA]]. | + | 1V1Q is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with CYS and CYS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V1Q OCA]]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: primosome]] | [[Category: primosome]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:36:20 2007'' |
Revision as of 10:31, 30 October 2007
|
CRYSTAL STRUCTURE OF PRIB- A PRIMOSOMAL DNA REPLICATION PROTEIN OF ESCHERICHIA COLI
Overview
PriB is one of the Escherichia coli varphiX-type primosome proteins that, are required for assembly of the primosome, a mobile multi-enzyme complex, responsible for the initiation of DNA replication. Here we report the, crystal structure of the E. coli PriB at 2.1 A resolution by, multi-wavelength anomalous diffraction using a mercury derivative. The, polypeptide chain of PriB is structurally similar to that of, single-stranded DNA-binding protein (SSB). However, the biological unit of, PriB is a dimer, not a homotetramer like SSB. Electrophoretic mobility, shift assays demonstrated that PriB binds single-stranded DNA and, single-stranded RNA with comparable affinity. We also show that PriB binds, single-stranded DNA with certain base preferences. Based on the PriB, structural information ... [(full description)]
About this Structure
1V1Q is a [Single protein] structure of sequence from [Escherichia coli] with CYS and CYS as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of PriB, a primosomal DNA replication protein of Escherichia coli., Liu JH, Chang TW, Huang CY, Chen SU, Wu HN, Chang MC, Hsiao CD, J Biol Chem. 2004 Nov 26;279(48):50465-71. Epub 2004 Sep 21. PMID:15383524
Page seeded by OCA on Tue Oct 30 12:36:20 2007
Categories: Escherichia coli | Single protein | Chang, M.C. | Chang, T.W. | Chen, S.U. | Hsiao, C.D. | Huang, C.Y. | Liu, J.H. | Wu, H.N. | CYS | Dna binding | Dna replication | Primosome
