1v11
From Proteopedia
(New page: 200px<br /> <applet load="1v11" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v11, resolution 1.95Å" /> '''CROSSTALK BETWEEN C...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1V11 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with K, MN, CL, TDP, BEN and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.4.4 1.2.4.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V11 OCA]]. | + | 1V11 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with K, MN, CL, TDP, BEN and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/3-methyl-2-oxobutanoate_dehydrogenase_(2-methylpropanoyl-transferring) 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.4.4 1.2.4.4]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V11 OCA]]. |
==Reference== | ==Reference== | ||
Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenase., Li J, Wynn RM, Machius M, Chuang JL, Karthikeyan S, Tomchick DR, Chuang DT, J Biol Chem. 2004 Jul 30;279(31):32968-78. Epub 2004 May 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15166214 15166214] | Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenase., Li J, Wynn RM, Machius M, Chuang JL, Karthikeyan S, Tomchick DR, Chuang DT, J Biol Chem. 2004 Jul 30;279(31):32968-78. Epub 2004 May 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15166214 15166214] | ||
| + | [[Category: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: thiamine phosphate]] | [[Category: thiamine phosphate]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:37:41 2007'' |
Revision as of 10:32, 30 October 2007
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CROSSTALK BETWEEN COFACTOR BINDING AND THE PHOSPHORYLATION LOOP CONFORMATION IN THE BCKD MACHINE
Overview
The decarboxylase/dehydrogenase (E1b) component of the 4-megadalton human, branched-chain alpha-keto acid dehydrogenase (BCKD) metabolic machine is a, thiamin diphosphate (ThDP)-dependent enzyme with a heterotetrameric, cofactor-binding fold. The E1b component catalyzes the decarboxylation of, alpha-keto acids and the subsequent reductive acylation of the lipoic, acid-bearing domain (LBD) from the 24-meric transacylase (E2b) core. In, the present study, we show that the binding of cofactor ThDP to the E1b, active site induces a disorder-to-order transition of the conserved, phosphorylation loop carrying the two phosphorylation sites Ser(292)-alpha, and Ser(302)-alpha, as deduced from the 1.80-1.85 A apoE1b and holoE1b, structures. The induced loop conformation is essential for the ... [(full description)]
About this Structure
1V11 is a [Protein complex] structure of sequences from [Homo sapiens] with K, MN, CL, TDP, BEN and GOL as [ligands]. Active as [3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)], with EC number [1.2.4.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenase., Li J, Wynn RM, Machius M, Chuang JL, Karthikeyan S, Tomchick DR, Chuang DT, J Biol Chem. 2004 Jul 30;279(31):32968-78. Epub 2004 May 27. PMID:15166214
Page seeded by OCA on Tue Oct 30 12:37:41 2007
Categories: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) | Homo sapiens | Protein complex | Chuang, D.T. | Chuang, J.L. | Karthikeyan, S. | Li, J. | Machius, M. | Tomchick, D.R. | Wynn, R.M. | BEN | CL | GOL | K | MN | TDP | Acylation | Branched-chain | Ketoacid dehydrogenase | Maple syrup urine disease | Multi-enzyme complex | Oxidative decarboxylation | Oxidoreductase | Phosphorylation | Thiamine phosphate
