1vz3
From Proteopedia
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| - | [[Image:1vz3.jpg|left|200px]]<br /><applet load="1vz3" size=" | + | [[Image:1vz3.jpg|left|200px]]<br /><applet load="1vz3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vz3, resolution 1.6Å" /> | caption="1vz3, resolution 1.6Å" /> | ||
'''PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, T597C MUTANT'''<br /> | '''PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, T597C MUTANT'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1VZ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] Known structural/functional Site: <scene name='pdbsite=AS1:Engineered Disulphide Bridge'>AS1</scene>. Full crystallographic information is available from [http:// | + | 1VZ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] Known structural/functional Site: <scene name='pdbsite=AS1:Engineered+Disulphide+Bridge'>AS1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZ3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:17:24 2008'' |
Revision as of 08:17, 3 February 2008
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PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, T597C MUTANT
Overview
Prolyl oligopeptidase contains a peptidase domain and its catalytic triad, is covered by the central tunnel of a seven-bladed beta-propeller. This, domain makes the enzyme an oligopeptidase by excluding large structured, peptides from the active site. The apparently rigid crystal structure does, not explain how the substrate can approach the catalytic groups. Two, possibilities of substrate access were investigated: either blades 1 and 7, of the propeller domain move apart, or the peptidase and/or propeller, domains move to create an entry site at the domain interface. Engineering, disulfide bridges to the expected oscillating structures prevented such, movements, which destroyed the catalytic activity and precluded substrate, binding. This indicated that concerted movements of the propeller and the, peptidase domains are essential for the enzyme action.
About this Structure
1VZ3 is a Single protein structure of sequence from Sus scrofa with as ligand. Active as Prolyl oligopeptidase, with EC number 3.4.21.26 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Concerted structural changes in the peptidase and the propeller domains of prolyl oligopeptidase are required for substrate binding., Szeltner Z, Rea D, Juhasz T, Renner V, Fulop V, Polgar L, J Mol Biol. 2004 Jul 9;340(3):627-37. PMID:15210359
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