1w2c
From Proteopedia
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| - | [[Image:1w2c.jpg|left|200px]]<br /><applet load="1w2c" size=" | + | [[Image:1w2c.jpg|left|200px]]<br /><applet load="1w2c" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w2c, resolution 1.95Å" /> | caption="1w2c, resolution 1.95Å" /> | ||
'''HUMAN INOSITOL (1,4,5) TRISPHOSPHATE 3-KINASE COMPLEXED WITH MN2+/AMPPNP/INS(1,4,5)P3'''<br /> | '''HUMAN INOSITOL (1,4,5) TRISPHOSPHATE 3-KINASE COMPLEXED WITH MN2+/AMPPNP/INS(1,4,5)P3'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W2C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MN, SO4, ANP and I3P as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.7.127 2.1.7.127] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W2C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=ANP:'>ANP</scene> and <scene name='pdbligand=I3P:'>I3P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.7.127 2.1.7.127] Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:18:25 2008'' |
Revision as of 08:18, 3 February 2008
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HUMAN INOSITOL (1,4,5) TRISPHOSPHATE 3-KINASE COMPLEXED WITH MN2+/AMPPNP/INS(1,4,5)P3
Overview
Mammalian cells produce a variety of inositol phosphates (InsPs), including Ins(1,4,5)P3 that serves both as a second messenger and as a, substrate for inositol polyphosphate kinases (IPKs), which further, phosphorylate it. We report the structure of an IPK, the human, Ins(1,4,5)P3 3-kinase-A, both free and in complexes with substrates and, products. This enzyme catalyzes transfer of a phosphate from ATP to the, 3-OH of Ins(1,4,5)P3, and its X-ray crystal structure provides a template, for understanding a broad family of InsP kinases. The catalytic domain, consists of three lobes. The N and C lobes bind ATP and resemble protein, and lipid kinases, despite insignificant sequence similarity. The third, lobe binds inositol phosphate and is a unique four-helix insertion in the, C lobe. This lobe embraces all of the phosphates of Ins(1,4,5)P3 in a, positively charged pocket, explaining the enzyme's substrate specificity, and its inability to phosphorylate PtdIns(4,5)P2, the membrane-resident, analog of Ins(1,4,5)P3.
About this Structure
1W2C is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Active as Transferase, with EC number 2.1.7.127 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of a human inositol 1,4,5-trisphosphate 3-kinase: substrate binding reveals why it is not a phosphoinositide 3-kinase., Gonzalez B, Schell MJ, Letcher AJ, Veprintsev DB, Irvine RF, Williams RL, Mol Cell. 2004 Sep 10;15(5):689-701. PMID:15350214
Page seeded by OCA on Sun Feb 3 10:18:25 2008
Categories: Homo sapiens | Single protein | Transferase | Gonzalez, B. | Irvine, R.F. | Schell, M.J. | Williams, R.L. | ANP | I3P | MN | SO4 | Amppnp | Inositol phosphate kinase | Ip3
