1w3o
From Proteopedia
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| - | [[Image:1w3o.jpg|left|200px]]<br /><applet load="1w3o" size=" | + | [[Image:1w3o.jpg|left|200px]]<br /><applet load="1w3o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w3o, resolution 1.60Å" /> | caption="1w3o, resolution 1.60Å" /> | ||
'''CRYSTAL STRUCTURE OF NIMA FROM D. RADIODURANS'''<br /> | '''CRYSTAL STRUCTURE OF NIMA FROM D. RADIODURANS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W3O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with ACT and PYR as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Pyr Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W3O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=PYR:'>PYR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Pyr+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W3O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: nim gene]] | [[Category: nim gene]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:18:56 2008'' |
Revision as of 08:18, 3 February 2008
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CRYSTAL STRUCTURE OF NIMA FROM D. RADIODURANS
Overview
5-Nitroimidazole-based antibiotics are compounds extensively used for, treating infections in humans and animals caused by several important, pathogens. They are administered as prodrugs, and their activation depends, upon an anaerobic 1-electron reduction of the nitro group by a reduction, pathway in the cells. Bacterial resistance toward these drugs is thought, to be caused by decreased drug uptake and/or an altered reduction, efficiency. One class of resistant strains, identified in Bacteroides, has, been shown to carry Nim genes (NimA, -B, -C, -D, and -E), which encode for, reductases that convert the nitro group on the antibiotic into a, non-bactericidal amine. In this paper, we have described the crystal, structure of NimA from Deinococcus radiodurans (drNimA) at 1.6 A, resolution. We have shown that drNimA is a homodimer in which each monomer, adopts a beta-barrel fold. We have identified the catalytically important, His-71 along with the cofactor pyruvate and antibiotic binding sites, all, of which are found at the monomer-monomer interface. We have reported, three additional crystal structures of drNimA, one in which the antibiotic, metronidazole is bound to the protein, one with pyruvate covalently bound, to His-71, and one with lactate covalently bound to His-71. Based on these, structures, a reaction mechanism has been proposed in which the 2-electron, reduction of the antibiotic prevents accumulation of the toxic nitro, radical. This mechanism suggests that Nim proteins form a new class of, reductases, conferring resistance against 5-nitroimidazole-based, antibiotics.
About this Structure
1W3O is a Single protein structure of sequence from Deinococcus radiodurans with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis of 5-nitroimidazole antibiotic resistance: the crystal structure of NimA from Deinococcus radiodurans., Leiros HK, Kozielski-Stuhrmann S, Kapp U, Terradot L, Leonard GA, McSweeney SM, J Biol Chem. 2004 Dec 31;279(53):55840-9. Epub 2004 Oct 18. PMID:15492014
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