1wc4
From Proteopedia
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| - | [[Image:1wc4.jpg|left|200px]]<br /><applet load="1wc4" size=" | + | [[Image:1wc4.jpg|left|200px]]<br /><applet load="1wc4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wc4, resolution 3.00Å" /> | caption="1wc4, resolution 3.00Å" /> | ||
'''SOLUBLE ADENYLYL CYCLASE CYAC FROM S. PLATENSIS IN COMPLEX WITH ALPHA,BETA-METHYLENE-ATP AND EUROPIUM'''<br /> | '''SOLUBLE ADENYLYL CYCLASE CYAC FROM S. PLATENSIS IN COMPLEX WITH ALPHA,BETA-METHYLENE-ATP AND EUROPIUM'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1WC4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrospira_platensis Arthrospira platensis] with EU3 and APC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] Known structural/functional Site: <scene name='pdbsite=AC1:Eu3 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1WC4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrospira_platensis Arthrospira platensis] with <scene name='pdbligand=EU3:'>EU3</scene> and <scene name='pdbligand=APC:'>APC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] Known structural/functional Site: <scene name='pdbsite=AC1:Eu3+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WC4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: soluble adenylyl cyclase]] | [[Category: soluble adenylyl cyclase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:21:39 2008'' |
Revision as of 08:21, 3 February 2008
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SOLUBLE ADENYLYL CYCLASE CYAC FROM S. PLATENSIS IN COMPLEX WITH ALPHA,BETA-METHYLENE-ATP AND EUROPIUM
Overview
In an evolutionarily conserved signaling pathway, 'soluble' adenylyl, cyclases (sACs) synthesize the ubiquitous second messenger cyclic, adenosine 3',5'-monophosphate (cAMP) in response to bicarbonate and, calcium signals. Here, we present crystal structures of a cyanobacterial, sAC enzyme in complex with ATP analogs, calcium and bicarbonate, which, represent distinct catalytic states of the enzyme. The structures reveal, that calcium occupies the first ion-binding site and directly mediates, nucleotide binding. The single ion-occupied, nucleotide-bound state, defines a novel, open adenylyl cyclase state. In contrast, bicarbonate, increases the catalytic rate by inducing marked active site closure and, recruiting a second, catalytic ion. The phosphates of the bound substrate, analogs are rearranged, which would facilitate product formation and, release. The mechanisms of calcium and bicarbonate sensing define a, reaction pathway involving active site closure and metal recruitment that, may be universal for class III cyclases.
About this Structure
1WC4 is a Single protein structure of sequence from Arthrospira platensis with and as ligands. Active as Adenylate cyclase, with EC number 4.6.1.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment., Steegborn C, Litvin TN, Levin LR, Buck J, Wu H, Nat Struct Mol Biol. 2005 Jan;12(1):32-7. Epub 2004 Dec 26. PMID:15619637
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