2a0b
From Proteopedia
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| - | [[Image:2a0b.jpg|left|200px]]<br /><applet load="2a0b" size=" | + | [[Image:2a0b.jpg|left|200px]]<br /><applet load="2a0b" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2a0b, resolution 1.57Å" /> | caption="2a0b, resolution 1.57Å" /> | ||
'''HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI'''<br /> | '''HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2A0B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=PHB:Phosphorylation Site'>PHB</scene> and <scene name='pdbsite=ZNB:Zn Binding Site'>ZNB</scene>. Full crystallographic information is available from [http:// | + | 2A0B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=PHB:Phosphorylation+Site'>PHB</scene> and <scene name='pdbsite=ZNB:Zn+Binding+Site'>ZNB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A0B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: two-component system]] | [[Category: two-component system]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:22:45 2008'' |
Revision as of 08:22, 3 February 2008
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HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI
Overview
The crystal structure of the histidine-containing phosphotransfer (HPt), domain of the anaerobic sensor kinase ArcB from Escherichia coli has been, refined to 1.57 A resolution, using the coordinates of the earlier 2.06 A, structure as a starting model. The final model contained 956 protein, atoms, one zinc ion and 156 water molecules, with an R factor of 19.0%., The high-resolution electron-density maps clearly revealed additional, solvent molecules and seven discrete rotamers in the protein side chains., One residue, Met755, was fully buried but was able to occupy the space in, the hydrophobic core by means of the two-state conformation of its side, chain. One water molecule was buried in the protein core and contributed, to the rigidity of the HPt domain, cooperating in the coordination of the, zinc ion.
About this Structure
2A0B is a Single protein structure of sequence from Escherichia coli with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Refined structure of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli at 1.57 A resolution., Kato M, Mizuno T, Shimizu T, Hakoshima T, Acta Crystallogr D Biol Crystallogr. 1999 Nov;55(Pt 11):1842-9. PMID:10531481
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