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2bfb
From Proteopedia
(New page: 200px<br /> <applet load="2bfb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bfb, resolution 1.77Å" /> '''REACTIVITY MODULATI...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BFB is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with K, MN, CL, TDP, MRD and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.4.4 1.2.4.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BFB OCA]]. | + | 2BFB is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with K, MN, CL, TDP, MRD and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/3-methyl-2-oxobutanoate_dehydrogenase_(2-methylpropanoyl-transferring) 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.4.4 1.2.4.4]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BFB OCA]]. |
==Reference== | ==Reference== | ||
A versatile conformational switch regulates reactivity in human branched-chain alpha-ketoacid dehydrogenase., Machius M, Wynn RM, Chuang JL, Li J, Kluger R, Yu D, Tomchick DR, Brautigam CA, Chuang DT, Structure. 2006 Feb;14(2):287-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16472748 16472748] | A versatile conformational switch regulates reactivity in human branched-chain alpha-ketoacid dehydrogenase., Machius M, Wynn RM, Chuang JL, Li J, Kluger R, Yu D, Tomchick DR, Brautigam CA, Chuang DT, Structure. 2006 Feb;14(2):287-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16472748 16472748] | ||
| + | [[Category: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: thiamine diphosphate]] | [[Category: thiamine diphosphate]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:45:25 2007'' |
Revision as of 10:40, 30 October 2007
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REACTIVITY MODULATION OF HUMAN BRANCHED-CHAIN ALPHA-KETOACID DEHYDROGENASE BY AN INTERNAL MOLECULAR SWITCH
Overview
The dehydrogenase/decarboxylase (E1b) component of the 4 MD human, branched-chain alpha-ketoacid dehydrogenase complex (BCKDC) is a thiamin, diphosphate (ThDP)-dependent enzyme. We have determined the crystal, structures of E1b with ThDP bound intermediates after decarboxylation of, alpha-ketoacids. We show that a key tyrosine residue in the E1b active, site functions as a conformational switch to reduce the reactivity of the, ThDP cofactor through interactions with its thiazolium ring. The, intermediates do not assume the often-postulated enamine state, but likely, a carbanion state. The carbanion presumably facilitates the second, E1b-catalyzed reaction, involving the transfer of an acyl moiety from the, intermediate to a lipoic acid prosthetic group in the transacylase (E2b), component ... [(full description)]
About this Structure
2BFB is a [Protein complex] structure of sequences from [Homo sapiens] with K, MN, CL, TDP, MRD and GOL as [ligands]. Active as [3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)], with EC number [1.2.4.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
A versatile conformational switch regulates reactivity in human branched-chain alpha-ketoacid dehydrogenase., Machius M, Wynn RM, Chuang JL, Li J, Kluger R, Yu D, Tomchick DR, Brautigam CA, Chuang DT, Structure. 2006 Feb;14(2):287-98. PMID:16472748
Page seeded by OCA on Tue Oct 30 12:45:25 2007
Categories: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) | Homo sapiens | Protein complex | Brautigam, C.A. | Chuang, D.T. | Chuang, J.L. | Machius, M. | Tomchick, D.R. | Wynn, R.M. | CL | GOL | K | MN | MRD | TDP | Acylation | Branched-chain | Conformational switch | Ketoacid dehydrogenase | Maple syrup urine disease | Multi-enzyme complex | Oxidative decarboxylation | Oxidoreductase | Phosphorylation | Reactivity | Thiamine diphosphate
