1gpu
From Proteopedia
(New page: 200px<br /> <applet load="1gpu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gpu, resolution 1.86Å" /> '''TRANSKETOLASE COMPL...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1GPU is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with CA and THD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]]. | + | 1GPU is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with CA and THD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transketolase Transketolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Transketolase]] | ||
[[Category: Fiedler, E.]] | [[Category: Fiedler, E.]] | ||
[[Category: Koenig, S.]] | [[Category: Koenig, S.]] | ||
| Line 23: | Line 24: | ||
[[Category: transferase(ketone residues)]] | [[Category: transferase(ketone residues)]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:47:13 2007'' |
Revision as of 10:42, 30 October 2007
|
TRANSKETOLASE COMPLEX WITH REACTION INTERMEDIATE
Overview
Kinetic and spectroscopic data indicated that addition of the donor, substrate hydroxypyruvate to the thiamin diphosphate (ThDP)-dependent, enzyme transketolase (TK) led to the accumulation of the, alpha-carbanion/enamine of (alpha,beta-dihydroxyethyl) ThDP, the key, reaction intermediate in enzymatic thiamin catalysis. The, three-dimensional structure of this intermediate trapped in the active, site of yeast TK was determined to 1.9-A resolution by using, cryocrystallography. The electron density suggests a planar, alpha-carbanion/enamine intermediate having the E-configuration. The, reaction intermediate is firmly held in place through direct hydrogen, bonds to His-103 and His-481 and an indirect hydrogen bond via a water, molecule to His-69. The 4-NH(2) group of the amino-pyrimidine ... [(full description)]
About this Structure
1GPU is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with CA and THD as [ligands]. Active as [Transketolase], with EC number [2.2.1.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Snapshot of a key intermediate in enzymatic thiamin catalysis: crystal structure of the alpha-carbanion of (alpha,beta-dihydroxyethyl)-thiamin diphosphate in the active site of transketolase from Saccharomyces cerevisiae., Fiedler E, Thorell S, Sandalova T, Golbik R, Konig S, Schneider G, Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):591-5. Epub 2002 Jan 2. PMID:11773632
Page seeded by OCA on Tue Oct 30 12:47:13 2007
