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2zb9

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[[Category: transcription regulator]]
[[Category: transcription regulator]]
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Revision as of 06:13, 13 February 2008

Image:2zb9.jpg

2zb9, resolution 2.25Å

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Crystal structure of TetR family transcription regulator SCO0332

Overview

SCO0332 protein is a putative TetR-family transcriptional regulator from, Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was, determined at 2.25 A resolution by single-wavelength anomalous diffraction, (SAD) phasing using the S atoms of the native protein. SCO0332 contains a, helix-turn-helix (HTH) DNA-binding motif in its N-terminal region and, forms a homodimer. The overall structure of SCO0332 shows significant, similarity to other TetR-family regulators. A systematic evolution of, ligands by exponential enrichment (SELEX) analysis indicated that SCO0332, has sequence-specific DNA-binding ability and determined the position of, the operator element of SCO0332 on the chromosomal DNA of S. coelicolor., An electrophoretic mobility-shift assay (EMSA) showed that SCO0332 binds, to the operator sequence upstream of the sco0330 gene, which encodes a, putative short-chain oxidoreductase. These results suggest that SCO0332 is, a transcriptional repressor that regulates sco0330 gene expression.

About this Structure

2ZB9 is a Single protein structure of sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA.

Reference

Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor., Okada U, Kondo K, Hayashi T, Watanabe N, Yao M, Tamura T, Tanaka I, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):198-205. Epub 2008, Jan 16. PMID:18219120

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