2rfk

From Proteopedia

Revision as of 06:14, 13 February 2008

Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex

Overview

The most complex RNA pseudouridylases are H/ACA ribonucleoprotein, particles, which use a guide RNA for substrate capture and four proteins, (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we, report the three-dimensional structure of a catalytically deficient, archaeal enzyme complex (including the guide RNA and three of the four, essential proteins) bound to a substrate RNA. Extensive interactions of, Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked, guide-substrate RNA complex structure and position the substrate in, proximity of the Cbf5 catalytic center. Our structural and complementary, fluorescence analyses also indicate that precise placement of the target, uridine at the active site requires a conformation of the guide-substrate, RNA duplex that is brought about by the previously identified concurrent, interaction of the guide RNA with L7Ae and a composite Cbf5-Nop10 surface, and further identify a residue that is critical in this process.

About this Structure

2RFK is a Protein complex structure of sequences from Pyrococcus furiosus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex., Liang B, Xue S, Terns RM, Terns MP, Li H, Nat Struct Mol Biol. 2007 Dec 2;. PMID:18059286

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