2h1c
From Proteopedia
(New page: 200px<br /><applet load="2h1c" size="350" color="white" frame="true" align="right" spinBox="true" caption="2h1c, resolution 1.80Å" /> '''Crystal Structure of...) |
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caption="2h1c, resolution 1.80Å" /> | caption="2h1c, resolution 1.80Å" /> | ||
'''Crystal Structure of FitAcB from Neisseria gonorrhoeae'''<br /> | '''Crystal Structure of FitAcB from Neisseria gonorrhoeae'''<br /> | ||
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| + | ==Overview== | ||
| + | Neisseria gonorrhoeae is a sexually transmitted pathogen that initiates, infections in humans by adhering to the mucosal epithelium of the, urogenital tract. The bacterium then enters the apical region of the cell, and traffics across the cell to exit into the subepithelial matrix., Mutations in the fast intracellular trafficking (fitAB) locus cause the, bacteria to transit a polarized epithelial monolayer more quickly than the, wild-type parent and to replicate within cells at an accelerated rate., Here, we describe the crystal structure of the toxin-antitoxin, heterodimer, FitAB, bound to a high affinity 36-bp DNA fragment from the, fitAB promoter. FitA, the antitoxin, binds DNA through its, ribbon-helix-helix motif and is tethered to FitB, the toxin, to form a, heterodimer by the insertion of a four turn alpha-helix into an extensive, FitB hydrophobic pocket. FitB is composed of a PIN (PilT N terminus), domain, with a central, twisted, 5-stranded parallel beta-sheet that is, open on one side and flanked by five alpha-helices. FitB in the context of, the FitAB complex does not display nuclease activity against tested PIN, substrates. The FitAB complex points to the mechanism by which antitoxins, with RHH motifs can block the activity of toxins with PIN domains., Interactions between two FitB molecules result in the formation of a, tetramer of FitAB heterodimers, which binds to the 36-bp DNA fragment and, provides an explanation for how FitB enhances the DNA binding affinity of, FitA. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2H1C is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1C OCA]. | + | 2H1C is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Act+Binding+Site+For+Residue+A+140'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+201'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+A+202'>AC3</scene>, <scene name='pdbsite=AC4:Mg+Binding+Site+For+Residue+A+203'>AC4</scene>, <scene name='pdbsite=AC5:Mg+Binding+Site+For+Residue+A+204'>AC5</scene> and <scene name='pdbsite=AC6:Mg+Binding+Site+For+Residue+A+205'>AC6</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1C OCA]. |
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| + | ==Reference== | ||
| + | Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs., Mattison K, Wilbur JS, So M, Brennan RG, J Biol Chem. 2006 Dec 8;281(49):37942-51. Epub 2006 Sep 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16982615 16982615] | ||
[[Category: Neisseria gonorrhoeae]] | [[Category: Neisseria gonorrhoeae]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: dna binding]] | [[Category: dna binding]] | ||
| + | [[Category: gene regulation]] | ||
[[Category: pin domain]] | [[Category: pin domain]] | ||
[[Category: rhh domain]] | [[Category: rhh domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:17:21 2008'' |
Revision as of 06:17, 13 February 2008
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Crystal Structure of FitAcB from Neisseria gonorrhoeae
Overview
Neisseria gonorrhoeae is a sexually transmitted pathogen that initiates, infections in humans by adhering to the mucosal epithelium of the, urogenital tract. The bacterium then enters the apical region of the cell, and traffics across the cell to exit into the subepithelial matrix., Mutations in the fast intracellular trafficking (fitAB) locus cause the, bacteria to transit a polarized epithelial monolayer more quickly than the, wild-type parent and to replicate within cells at an accelerated rate., Here, we describe the crystal structure of the toxin-antitoxin, heterodimer, FitAB, bound to a high affinity 36-bp DNA fragment from the, fitAB promoter. FitA, the antitoxin, binds DNA through its, ribbon-helix-helix motif and is tethered to FitB, the toxin, to form a, heterodimer by the insertion of a four turn alpha-helix into an extensive, FitB hydrophobic pocket. FitB is composed of a PIN (PilT N terminus), domain, with a central, twisted, 5-stranded parallel beta-sheet that is, open on one side and flanked by five alpha-helices. FitB in the context of, the FitAB complex does not display nuclease activity against tested PIN, substrates. The FitAB complex points to the mechanism by which antitoxins, with RHH motifs can block the activity of toxins with PIN domains., Interactions between two FitB molecules result in the formation of a, tetramer of FitAB heterodimers, which binds to the 36-bp DNA fragment and, provides an explanation for how FitB enhances the DNA binding affinity of, FitA.
About this Structure
2H1C is a Protein complex structure of sequences from Neisseria gonorrhoeae with , and as ligands. Known structural/functional Sites: , , , , and . Full crystallographic information is available from OCA.
Reference
Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs., Mattison K, Wilbur JS, So M, Brennan RG, J Biol Chem. 2006 Dec 8;281(49):37942-51. Epub 2006 Sep 18. PMID:16982615
Page seeded by OCA on Wed Feb 13 08:17:21 2008
Categories: Neisseria gonorrhoeae | Protein complex | Brennan, R.G. | Mattison, K. | So, M. | Wilbur, J.S. | ACT | MG | SO4 | Dna binding | Gene regulation | Pin domain | Rhh domain
