1a9n
From Proteopedia
(New page: 200px<br /> <applet load="1a9n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a9n, resolution 2.38Å" /> '''CRYSTAL STRUCTURE O...) |
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caption="1a9n, resolution 2.38Å" /> | caption="1a9n, resolution 2.38Å" /> | ||
'''CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA'''<br /> | '''CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A9N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1A9N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A9N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: snrnp]] | [[Category: snrnp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:28:24 2008'' |
Revision as of 13:28, 15 February 2008
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CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA
Overview
We have determined the crystal structure at 2.4 A resolution of a ternary, complex between the spliceosomal U2B"/U2A' protein complex and, hairpin-loop IV of U2 small nuclear RNA. Unlike its close homologue the, U1A protein, U2B" binds to its cognate RNA only in the presence of U2A', which contains leucine-rich repeats in its sequence. The concave surface, of a parallel beta-sheet within the leucine-rich-repeat region of U2A', interacts with the ribonucleoprotein domain of U2B" on the surface, opposite its RNA-binding surface. The basic carboxy-terminal region of, U2A' interacts with the RNA stem. The crystal structure reveals how, protein-protein interaction regulates RNA-binding specificity, and how, replacing only a few key residues allows the U2B" and U1A proteins to, discriminate between their cognate RNA hairpins by forming alternative, networks of interactions.
About this Structure
1A9N is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the spliceosomal U2B"-U2A' protein complex bound to a fragment of U2 small nuclear RNA., Price SR, Evans PR, Nagai K, Nature. 1998 Aug 13;394(6694):645-50. PMID:9716128
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