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1ana

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(New page: 200px<br /> <applet load="1ana" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ana, resolution 2.000&Aring;" /> '''HELIX GEOMETRY AND...)
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'''HELIX GEOMETRY AND HYDRATION IN AN A-DNA TETRAMER. IC-C-G-G'''<br />
'''HELIX GEOMETRY AND HYDRATION IN AN A-DNA TETRAMER. IC-C-G-G'''<br />
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==About this Structure==
==About this Structure==
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1ANA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 1ANA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb23_1.html DNA]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ANA OCA].
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1ANA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 1ANA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb23_1.html DNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ANA OCA].
==Reference==
==Reference==
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 08:56:58 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:29:22 2008''

Revision as of 13:29, 15 February 2008

Image:1ana.jpg

1ana, resolution 2.000Å

Drag the structure with the mouse to rotate

HELIX GEOMETRY AND HYDRATION IN AN A-DNA TETRAMER. IC-C-G-G

Overview

The DNA oligomer of sequence IC-C-G-G has been synthesized, and its X-ray, crystal structure solved at a resolution of 2.0 A, using anomalous, scattering from iodines in phase analysis: 48 cycles of Jack-Levitt, restrained least-squares refinement resulted in a residual error of 19.9%, over all data, or 16.5% for two-sigma data. Two double-helical tetramers, stack in the crystal to form a continuous octamer, except for the two, missing phosphate connections across the center. The octamer has a mean, helix rotation of 33.7 degrees (10.7 base-pairs per turn), rise of 2.87 A, mean inclination angle of base-pairs of 14 degrees, and mean base-pair, propeller twist of +16.3 degrees. Local variations in both helix rotation, and base plane roll angles, including those across the center of the, octamer, are as predicted from base sequence by sum functions sigma 1 and, sigma 2. The three known DNA octamers: IC-C-G-G/IC-C-G-G, G-G-T-A-T-A-C-C, and G-G-C-C-G-G-C-C, make up a graded series in this order, with, monotonically changing structural parameters. An exhaustive comparison of, torsion angle correlations among the known A helices confirms some, structural expectations and reveals some new features. 86 water molecules, have been located per double-helical IC-C-G-G tetramer (the asymmetric, unit), of which 451/2 per tetramer lie within a first hydrogen-bonded, shell of hydration. No ordered water structure is observed comparable to, the minor groove spine of hydration in B-DNA.

About this Structure

1ANA is a Protein complex structure of sequences from [1]. The following page contains interesting information on the relation of 1ANA with [DNA]. Full crystallographic information is available from OCA.

Reference

Helix geometry and hydration in an A-DNA tetramer: IC-C-G-G., Conner BN, Yoon C, Dickerson JL, Dickerson RE, J Mol Biol. 1984 Apr 25;174(4):663-95. PMID:6726797

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