1ds6
From Proteopedia
(New page: 200px<br /> <applet load="1ds6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ds6, resolution 2.35Å" /> '''CRYSTAL STRUCTURE O...) |
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caption="1ds6, resolution 2.35Å" /> | caption="1ds6, resolution 2.35Å" /> | ||
'''CRYSTAL STRUCTURE OF A RAC-RHOGDI COMPLEX'''<br /> | '''CRYSTAL STRUCTURE OF A RAC-RHOGDI COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1DS6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1DS6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DS6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: walker fold]] | [[Category: walker fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:40:43 2008'' |
Revision as of 13:40, 15 February 2008
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CRYSTAL STRUCTURE OF A RAC-RHOGDI COMPLEX
Contents |
Overview
Rho family-specific guanine nucleotide dissociation inhibitors (RhoGDIs), decrease the rate of nucleotide dissociation and release Rho proteins such, as RhoA, Rac and Cdc42 from membranes, forming tight complexes that, shuttle between cytosol and membrane compartments. We have solved the, crystal structure of a complex between the RhoGDI homolog LyGDI and, GDP-bound Rac2, which are abundant in leukocytes, representing the, cytosolic, resting pool of Rho species to be activated by extracellular, signals. The N-terminal domain of LyGDI (LyN), which has been reported to, be flexible in isolated RhoGDIs, becomes ordered upon complex formation, and contributes more than 60% to the interface area. The structure is, consistent with the C-terminus of Rac2 binding to a hydrophobic cavity, previously proposed as isoprenyl binding site. An inner segment of LyN, forms a helical hairpin that contacts mainly the switch regions of Rac2., The architecture of the complex interface suggests a mechanism for the, inhibition of guanine nucleotide dissociation that is based on the, stabilization of the magnesium (Mg2+) ion in the nucleotide binding, pocket.
Disease
Known disease associated with this structure: Neutrophil immunodeficiency syndrome OMIM:[602049]
About this Structure
1DS6 is a Protein complex structure of sequences from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
The Rac-RhoGDI complex and the structural basis for the regulation of Rho proteins by RhoGDI., Scheffzek K, Stephan I, Jensen ON, Illenberger D, Gierschik P, Nat Struct Biol. 2000 Feb;7(2):122-6. PMID:10655614
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