This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1fyh
From Proteopedia
(New page: 200px<br /> <applet load="1fyh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fyh, resolution 2.04Å" /> '''1:1 COMPLEX BETWEEN...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1fyh. | + | [[Image:1fyh.jpg|left|200px]]<br /><applet load="1fyh" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1fyh" size=" | + | |
caption="1fyh, resolution 2.04Å" /> | caption="1fyh, resolution 2.04Å" /> | ||
'''1:1 COMPLEX BETWEEN AN INTERFERON GAMMA SINGLE-CHAIN VARIANT AND ITS RECEPTOR'''<br /> | '''1:1 COMPLEX BETWEEN AN INTERFERON GAMMA SINGLE-CHAIN VARIANT AND ITS RECEPTOR'''<br /> | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1FYH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1FYH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FYH OCA]. |
==Reference== | ==Reference== | ||
| Line 23: | Line 22: | ||
[[Category: fibronectin type-iii]] | [[Category: fibronectin type-iii]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:49:52 2008'' |
Revision as of 13:49, 15 February 2008
|
1:1 COMPLEX BETWEEN AN INTERFERON GAMMA SINGLE-CHAIN VARIANT AND ITS RECEPTOR
Contents |
Overview
BACKGROUND: Interferon-gamma (IFN-gamma) is a homodimeric cytokine that, exerts its various activities by inducing the aggregation of two different, receptors. The alpha chain receptor (IFN-gammaRalpha) is a high affinity, receptor that binds to IFN-gamma in a symmetric bivalent manner to form a, stable, intermediate 1:2 complex. This intermediate forms a binding, template for the subsequent binding of two copies of the second receptor, beta chain receptor (IFN-gammaRbeta), producing the active 1:2:2 signaling, complex. RESULTS: A single chain monovalent variant of IFN-gamma, (scIFN-gamma) was constructed and complexed to one copy of the, extracellular domain (ECD) of IFN-gammaRalpha. The structure of this 1:1, complex was determined and the hormone-receptor interface shown to be, characterized by a number of hydrophilic interactions mediated by several, highly ordered water networks. The scIFN-gamma interface consists of, segments from each of the monomer chains of the homodimer. The principal, hydrophobic contact of the receptor involves a tripeptide segment of the, receptor having an unusual and high energy conformation. Despite, containing only one binding site for IFN-gammaRalpha, the monovalent, scIFN-gamma molecule has significant activity in antiviral biological, assays. CONCLUSIONS: ScIFN-gamma binds the ECD of IFN-gammaRalpha through, a highly hydrated interface with an important set of hormone-receptor, contacts mediated through structured waters. Although the interface is, highly hydrated, it supports tight binding and has a considerable degree, of specificity. The biological activity of scIFN-gamma confirms that the, scIFN-gamma:IFN-gammaRalpha complex represents a productive intermediate, and that it can effectively recruit the other required component, IFN-gammaRbeta, to signal based on the 1:1:1 complex.
Disease
Known diseases associated with this structure: AIDS, rapid progression to OMIM:[147570], Aplastic anemia OMIM:[147570], BCG infection, generalized familial OMIM:[107470], H. pylori infection, susceptibility to OMIM:[107470], Hepatitis C virus, resistance to OMIM:[147570], Interferon, immune, deficiency OMIM:[147570], Mycobacterial infection, atypical, familial disseminated OMIM:[107470], TSC2 angiomyolipomas, renal, modifier of OMIM:[147570], Tuberculosis, susceptibility to OMIM:[107470], Tuberculosis, susceptibility to OMIM:[147570]
About this Structure
1FYH is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
The structure and activity of a monomeric interferon-gamma:alpha-chain receptor signaling complex., Randal M, Kossiakoff AA, Structure. 2001 Feb 7;9(2):155-63. PMID:11250200
Page seeded by OCA on Fri Feb 15 15:49:52 2008
