1ldr
From Proteopedia
(New page: 200px<br /> <applet load="1ldr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ldr" /> '''SECOND REPEAT OF THE LDL RECEPTOR LIGAND-BI...) |
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'''SECOND REPEAT OF THE LDL RECEPTOR LIGAND-BINDING DOMAIN'''<br /> | '''SECOND REPEAT OF THE LDL RECEPTOR LIGAND-BINDING DOMAIN'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LDR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1LDR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ldl receptor cysteine-rich repeat]] | [[Category: ldl receptor cysteine-rich repeat]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:17:36 2008'' |
Revision as of 14:17, 15 February 2008
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SECOND REPEAT OF THE LDL RECEPTOR LIGAND-BINDING DOMAIN
Contents |
Overview
The ligand-binding domain of the low-density lipoprotein receptor, comprises seven cysteine-rich repeats, which have been highly conserved, through evolution. This domain mediates interactions of the receptor with, two lipoprotein apoproteins, apo E and apo B-100, putatively through a, calcium-dependent association of the ligands with a cluster of acidic, residues on the receptor. The second repeat (rLB2) of the receptor binding, domain has been expressed as a thrombin-cleavable GST fusion protein, cleaved, and purified. On oxidation the protein refolded to give a single, peak on reverse-phase HPLC. The aqueous solution structure of rLB2 has, been determined using two-dimensional 1H NMR spectroscopy. In contrast to, the amino-terminal repeat, rLB1, rLB2 has a very flexible structure in, water. However, the conformation of rLB2 is markedly more ordered in the, presence of a 4-fold molar excess of calcium chloride; the proton, resonance dispersion and the number of NOESY cross-peaks are greatly, enhanced. The three-dimensional structure of rLB2, obtained from the NMR, data by molecular geometry and restrained molecular dynamics methods, parallels that of rLB1, with an amino-terminal hairpin structure followed, by a succession of turns. However, there are clear differences in the, backbone topology and structural flexibility. As for rLB1, the acidic, residues are clustered on one face of the module. The side chain of Asp, 37, which is part of a completely conserved SDE sequence thought to be, involved in ligand binding, is buried, as is its counterpart (Asp 36) in, rLB1. These results provide the first experimental support for the, hypothesis that each of the repeats in the ligand-binding domain has a, similar global fold but also highlight significant differences in, structure and internal dynamics.
Disease
Known disease associated with this structure: Hypercholesterolemia, familial OMIM:[606945]
About this Structure
1LDR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the second cysteine-rich repeat from the human low-density lipoprotein receptor., Daly NL, Djordjevic JT, Kroon PA, Smith R, Biochemistry. 1995 Nov 7;34(44):14474-81. PMID:7578052
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