1qbv

From Proteopedia

Revision as of 14:44, 15 February 2008

CRYSTAL STRUCTURE OF THROMBIN COMPLEXED WITH AN GUANIDINE-MIMETIC INHIBITOR

Overview

The structure of the noncovalent complex of human alpha-thrombin with a, nonpeptide inhibitor containing a central phenyl scaffold, N-[2-[5-methyl-3-(2-chlorophenylsulfonyloxy)phenoxy]ethyl]-N- methyl-4, -aminopyridine (1), has been determined to 2.20 A resolution. In addition, the thrombin-bound structures of two distinct amino acid-based inhibitors, (3 and 4) containing different aminopyridine-derived guanidine mimetics, have been determined. Each compound occupies the same region of the active, site and projects an aminopyridine, a central hydrophobic group, and an, aryl group, into the S1, S2, and aryl subsites on thrombin. Nonpeptide 1, forms only one direct intermolecular hydrogen bond to the thrombin active, site and forms no hydrogen bonds to ordered molecules of solvent. Close, contacts are observed between main-chain carbonyl groups on thrombin and, the edges of the central phenyl and aminopyridine rings and the sulfonyl, group of 1 such that atoms carrying opposite partial charges are, juxtaposed. Aminopyridine groups in 3 and 4 also form close contacts with, the edges of carbonyl groups on thrombin and are flexibly accommodated in, the S1 subsite. Superposition of the bound conformations of 1 and, D-Phe-Pro-amidobutylguanidine (2) revealed that the central phenyl, scaffold of 1 substitutes for the peptide main chain of 2.

Disease

Known diseases associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]

About this Structure

1QBV is a Protein complex structure of sequences from Hirudinaria manillensis and Homo sapiens with as ligand. Active as Thrombin, with EC number 3.4.21.5 Full crystallographic information is available from OCA.

Reference

Structural analysis of thrombin complexed with potent inhibitors incorporating a phenyl group as a peptide mimetic and aminopyridines as guanidine substitutes., Bone R, Lu T, Illig CR, Soll RM, Spurlino JC, J Med Chem. 1998 Jun 4;41(12):2068-75. PMID:9622548

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