This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1r4x
From Proteopedia
(New page: 200px<br /> <applet load="1r4x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r4x, resolution 1.90Å" /> '''Crystal Structure A...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1r4x. | + | [[Image:1r4x.jpg|left|200px]]<br /><applet load="1r4x" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1r4x" size=" | + | |
caption="1r4x, resolution 1.90Å" /> | caption="1r4x, resolution 1.90Å" /> | ||
'''Crystal Structure Analys of the Gamma-COPI Appendage domain'''<br /> | '''Crystal Structure Analys of the Gamma-COPI Appendage domain'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1R4X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1R4X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4X OCA]. |
==Reference== | ==Reference== | ||
| Line 22: | Line 21: | ||
[[Category: appendage; beta sandwich; coatomer; adp-ribosylation factors]] | [[Category: appendage; beta sandwich; coatomer; adp-ribosylation factors]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:46:55 2008'' |
Revision as of 14:46, 15 February 2008
|
Crystal Structure Analys of the Gamma-COPI Appendage domain
Overview
COPI-coated vesicles mediate retrograde transport from the Golgi back to, the ER and intra-Golgi transport. The cytosolic precursor of the COPI, coat, the heptameric coatomer complex, can be thought of as composed of, two subcomplexes. The first consists of the beta-, gamma-, delta- and, zeta-COP subunits which are distantly homologous to AP clathrin adaptor, subunits. The second consists of the alpha-, beta'- and epsilon-COP, subunits. Here, we present the structure of the appendage domain of, gamma-COP and show that it has a similar overall fold as the, alpha-appendage of AP2. Again, like the alpha-appendage the gamma-COP, appendage possesses a single protein/protein interaction site on its, platform subdomain. We show that in yeast this site binds to the ARFGAP, Glo3p, and in mammalian gamma-COP this site binds to a Glo3p orthologue, ARFGAP2. On the basis of mutations in the yeast homologue of gamma-COP, Sec21p, a second binding site is proposed to exist on the gamma-COP, appendage that interacts with the alpha,beta',epsilon COPI subcomplex.
About this Structure
1R4X is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Gamma-COP appendage domain - structure and function., Watson PJ, Frigerio G, Collins BM, Duden R, Owen DJ, Traffic. 2004 Feb;5(2):79-88. PMID:14690497
Page seeded by OCA on Fri Feb 15 16:46:55 2008
