1ab9
From Proteopedia
(New page: 200px<br /> <applet load="1ab9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ab9, resolution 1.60Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1AB9 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AB9 OCA]]. | + | 1AB9 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AB9 OCA]]. |
==Reference== | ==Reference== | ||
X-ray crystal structure of a dipeptide-chymotrypsin complex in an inhibitory interaction., Kashima A, Inoue Y, Sugio S, Maeda I, Nose T, Shimohigashi Y, Eur J Biochem. 1998 Jul 1;255(1):12-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9692896 9692896] | X-ray crystal structure of a dipeptide-chymotrypsin complex in an inhibitory interaction., Kashima A, Inoue Y, Sugio S, Maeda I, Nose T, Shimohigashi Y, Eur J Biochem. 1998 Jul 1;255(1):12-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9692896 9692896] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
| + | [[Category: Chymotrypsin]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Inoue, Y.]] | [[Category: Inoue, Y.]] | ||
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[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:18:56 2007'' |
Revision as of 11:14, 30 October 2007
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CRYSTAL STRUCTURE OF BOVINE GAMMA-CHYMOTRYPSIN
Overview
The dipeptide D-leucyl-L-phenylalanyl p-fluorobenzylamide, (D-Leu-Phe-NH-BzlF) inhibits chymotrypsin strongly in a competitive manner, with the Ki value of 0.61 microM [Shimohigashi, Y., Maeda, I., Nose, T., Ikesue, K., Sakamoto, H., Ogawa, T., Ide, Y., Kawahara, M., Nezu, T., Terada, Y., Kawano, K. & Ohno, M. (1996) J. Chem. Soc. Perkin Trans. 1, 2479-2485]. The structure/activity studies have suggested a unique, inhibitory conformation, in which the C-terminal benzyl group fits the, chymotrypsin S1 site and the hydrophobic core constructed by the side, chains of D-Leu-Phe fits the S2 or S1' site. To verify this assumption, the molecular structure of the complex between the dipeptide and, gamma-chymotrypsin has been determined crystallographically., Gamma-chymotrypsin itself was ... [(full description)]
About this Structure
1AB9 is a [Protein complex] structure of sequences from [Bos taurus] with SO4 as [ligand]. Active as [Chymotrypsin], with EC number [3.4.21.1]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
X-ray crystal structure of a dipeptide-chymotrypsin complex in an inhibitory interaction., Kashima A, Inoue Y, Sugio S, Maeda I, Nose T, Shimohigashi Y, Eur J Biochem. 1998 Jul 1;255(1):12-23. PMID:9692896
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