2dts
From Proteopedia
(New page: 200px<br /> <applet load="2dts" size="450" color="white" frame="true" align="right" spinBox="true" caption="2dts, resolution 2.20Å" /> '''Crystal Structure o...) |
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caption="2dts, resolution 2.20Å" /> | caption="2dts, resolution 2.20Å" /> | ||
'''Crystal Structure of the Defucosylated Fc Fragment from Human Immunoglobulin G1'''<br /> | '''Crystal Structure of the Defucosylated Fc Fragment from Human Immunoglobulin G1'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2DTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DTS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
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Revision as of 15:22, 15 February 2008
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Crystal Structure of the Defucosylated Fc Fragment from Human Immunoglobulin G1
Overview
Removal of the fucose residue from the oligosaccharides attached to Asn297, of human immunoglobulin G1 (IgG1) results in a significant enhancement of, antibody-dependent cellular cytotoxicity (ADCC) via improved IgG1 binding, to Fcgamma receptor IIIa. To provide structural insight into the, mechanisms of affinity enhancement, we determined the crystal structure of, the nonfucosylated Fc fragment and compared it with that of fucosylated, Fc. The overall conformations of the fucosylated and nonfucosylated Fc, fragments were similar except for hydration mode around Tyr296., Stable-isotope-assisted NMR analyses confirmed the similarity of the, overall structures between fucosylated and nonfucosylated Fc fragments in, solution. These data suggest that the glycoform-dependent ADCC enhancement, is attributed to a subtle conformational alteration in a limited region of, IgG1-Fc. Furthermore, the electron density maps revealed that the traces, between Asp280 and Asn297 of our fucosylated and nonfucosylated Fc, crystals were both different from that in previously reported isomorphous, Fc crystals.
About this Structure
2DTS is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structural comparison of fucosylated and nonfucosylated fc fragments of human immunoglobulin g1., Matsumiya S, Yamaguchi Y, Saito J, Nagano M, Sasakawa H, Otaki S, Satoh M, Shitara K, Kato K, J Mol Biol. 2007 May 4;368(3):767-79. Epub 2007 Feb 22. PMID:17368483
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Categories: Homo sapiens | Single protein | Kato, K. | Matsumiya, S. | Nagano, M. | Otaki, S. | Saito, J. | Sasakawa, H. | Satoh, M. | Shitara, K. | Yamaguchi, Y. | NAG | Defucosylated glycoform | Igg1 | Immunoglobulin
