111d
From Proteopedia
(New page: 200px<br /><applet load="111d" size="450" color="white" frame="true" align="right" spinBox="true" caption="111d, resolution 2.250Å" /> '''CRYSTAL STRUCTURE A...) |
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| - | [[Image:111d.jpg|left|200px]]<br /><applet load="111d" size=" | + | [[Image:111d.jpg|left|200px]]<br /><applet load="111d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="111d, resolution 2.250Å" /> | caption="111d, resolution 2.250Å" /> | ||
'''CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS'''<br /> | '''CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by | + | The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by single-crystal X-ray diffraction techniques and the structure refined to R = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules. The sequence crystallizes as a full turn of a B-DNA helix with ten Watson-Crick base-pairs and two adenine-guanine mispairs. The analysis clearly shows that the mismatches are of the form A(anti).G(syn). Thermal denaturation studies indicate that the stability of the duplex is strongly pH dependent, with a maximum at pH 5.0, suggesting that the base-pair is stabilized by protonation. Three different arrangements have been observed for base-pairs between guanine and adenine and it is likely that A.G mismatch conformation is strongly influenced by dipole-dipole interactions with adjacent base-pairs. |
==About this Structure== | ==About this Structure== | ||
| - | 111D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 111D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=111D OCA]. |
==Reference== | ==Reference== | ||
Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs., Brown T, Leonard GA, Booth ED, Chambers J, J Mol Biol. 1989 May 20;207(2):455-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2754734 2754734] | Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs., Brown T, Leonard GA, Booth ED, Chambers J, J Mol Biol. 1989 May 20;207(2):455-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2754734 2754734] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Booth, E | + | [[Category: Booth, E D.]] |
[[Category: Brown, T.]] | [[Category: Brown, T.]] | ||
[[Category: Chambers, J.]] | [[Category: Chambers, J.]] | ||
| - | [[Category: Leonard, G | + | [[Category: Leonard, G A.]] |
[[Category: b-dna]] | [[Category: b-dna]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: mismatched]] | [[Category: mismatched]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:37:34 2008'' |
Revision as of 09:37, 21 February 2008
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CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS
Overview
The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by single-crystal X-ray diffraction techniques and the structure refined to R = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules. The sequence crystallizes as a full turn of a B-DNA helix with ten Watson-Crick base-pairs and two adenine-guanine mispairs. The analysis clearly shows that the mismatches are of the form A(anti).G(syn). Thermal denaturation studies indicate that the stability of the duplex is strongly pH dependent, with a maximum at pH 5.0, suggesting that the base-pair is stabilized by protonation. Three different arrangements have been observed for base-pairs between guanine and adenine and it is likely that A.G mismatch conformation is strongly influenced by dipole-dipole interactions with adjacent base-pairs.
About this Structure
111D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs., Brown T, Leonard GA, Booth ED, Chambers J, J Mol Biol. 1989 May 20;207(2):455-7. PMID:2754734
Page seeded by OCA on Thu Feb 21 11:37:34 2008
