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1a7s
From Proteopedia
(New page: 200px<br /> <applet load="1a7s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a7s, resolution 1.12Å" /> '''ATOMIC RESOLUTION S...) |
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| - | [[Image:1a7s.gif|left|200px]]<br /> | + | [[Image:1a7s.gif|left|200px]]<br /><applet load="1a7s" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1a7s" size=" | + | |
caption="1a7s, resolution 1.12Å" /> | caption="1a7s, resolution 1.12Å" /> | ||
'''ATOMIC RESOLUTION STRUCTURE OF HBP'''<br /> | '''ATOMIC RESOLUTION STRUCTURE OF HBP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Crystals of human heparin binding protein (HBP) diffract to 1.1 A when | + | Crystals of human heparin binding protein (HBP) diffract to 1.1 A when flash-frozen at 120 K. The atomic resolution structure has been refined anisotropically using SHELXL96. The final model of HBP consists of 221 amino-acid residues of 225 possible, three glycosylation units, one chloride ion, 15 precipitant ethanol molecules and 323 water molecules. The structure is refined to a final crystallographic R factor of 15.9% and Rfree(5%) of 18.9% using all data. A putative protein kinase C activation site has been identified, involving residues 113-120. The structure is compared to the previously determined 2.3 A resolution structure of HBP. |
==About this Structure== | ==About this Structure== | ||
| - | 1A7S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG, CL and EOH as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1A7S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=EOH:'>EOH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Flodgaard, H | + | [[Category: Flodgaard, H J.]] |
| - | [[Category: Iversen, L | + | [[Category: Iversen, L F.]] |
[[Category: Karlsen, S.]] | [[Category: Karlsen, S.]] | ||
| - | [[Category: Kastrup, J | + | [[Category: Kastrup, J S.]] |
| - | [[Category: Larsen, I | + | [[Category: Larsen, I K.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: EOH]] | [[Category: EOH]] | ||
| Line 26: | Line 25: | ||
[[Category: serine protease homolog]] | [[Category: serine protease homolog]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:49 2008'' |
Revision as of 09:41, 21 February 2008
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ATOMIC RESOLUTION STRUCTURE OF HBP
Overview
Crystals of human heparin binding protein (HBP) diffract to 1.1 A when flash-frozen at 120 K. The atomic resolution structure has been refined anisotropically using SHELXL96. The final model of HBP consists of 221 amino-acid residues of 225 possible, three glycosylation units, one chloride ion, 15 precipitant ethanol molecules and 323 water molecules. The structure is refined to a final crystallographic R factor of 15.9% and Rfree(5%) of 18.9% using all data. A putative protein kinase C activation site has been identified, involving residues 113-120. The structure is compared to the previously determined 2.3 A resolution structure of HBP.
About this Structure
1A7S is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
Atomic resolution structure of human HBP/CAP37/azurocidin., Karlsen S, Iversen LF, Larsen IK, Flodgaard HJ, Kastrup JS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):598-609. PMID:9761855
Page seeded by OCA on Thu Feb 21 11:41:49 2008
