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1alu
From Proteopedia
(New page: 200px<br /> <applet load="1alu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1alu, resolution 1.9Å" /> '''HUMAN INTERLEUKIN-6'...) |
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| - | [[Image:1alu.gif|left|200px]]<br /> | + | [[Image:1alu.gif|left|200px]]<br /><applet load="1alu" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1alu" size=" | + | |
caption="1alu, resolution 1.9Å" /> | caption="1alu, resolution 1.9Å" /> | ||
'''HUMAN INTERLEUKIN-6'''<br /> | '''HUMAN INTERLEUKIN-6'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Interleukin 6 (IL-6) has many biological activities in vivo, and | + | Interleukin 6 (IL-6) has many biological activities in vivo, and deregulation has been implicated in many disease processes. IL-6, a 185 amino acid polypeptide was refolded, purified and crystallized. The crystals diffracted to beyond 1.9 A and the structure was solved using single isomorphous replacement. The X-ray structure of IL-6 is composed of a four helix bundle linked by loops and an additional mini-helix. 157 out of 185 residues are well defined in the final structure, with 18 N-terminal and 8 A-B loop amino acids displaying no interpretable electron density. The three-dimensional structure has been used to construct a model of IL-6 interacting with the IL-6 receptor (alpha-chain) and gp130 (beta-chain) that gives new insight into the process of molecular recognition and signaling. Based on this model, we predict a fourth binding site on IL-6, a low affinity IL-6-IL-6 interaction, which may be necessary for the sequential assembly of a functional hexameric IL-6 receptor complex. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ALU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and TLA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1ALU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=TLA:'>TLA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ALU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Seehra, J | + | [[Category: Seehra, J S.]] |
| - | [[Category: Somers, W | + | [[Category: Somers, W S.]] |
[[Category: Stahl, M.]] | [[Category: Stahl, M.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
| Line 28: | Line 27: | ||
[[Category: signaling]] | [[Category: signaling]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:45:56 2008'' |
Revision as of 09:45, 21 February 2008
|
HUMAN INTERLEUKIN-6
Contents |
Overview
Interleukin 6 (IL-6) has many biological activities in vivo, and deregulation has been implicated in many disease processes. IL-6, a 185 amino acid polypeptide was refolded, purified and crystallized. The crystals diffracted to beyond 1.9 A and the structure was solved using single isomorphous replacement. The X-ray structure of IL-6 is composed of a four helix bundle linked by loops and an additional mini-helix. 157 out of 185 residues are well defined in the final structure, with 18 N-terminal and 8 A-B loop amino acids displaying no interpretable electron density. The three-dimensional structure has been used to construct a model of IL-6 interacting with the IL-6 receptor (alpha-chain) and gp130 (beta-chain) that gives new insight into the process of molecular recognition and signaling. Based on this model, we predict a fourth binding site on IL-6, a low affinity IL-6-IL-6 interaction, which may be necessary for the sequential assembly of a functional hexameric IL-6 receptor complex.
Disease
Known diseases associated with this structure: Kaposi sarcoma, susceptibility to OMIM:[147620], Osteopenia/osteoporosis OMIM:[147620]
About this Structure
1ALU is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
1.9 A crystal structure of interleukin 6: implications for a novel mode of receptor dimerization and signaling., Somers W, Stahl M, Seehra JS, EMBO J. 1997 Mar 3;16(5):989-97. PMID:9118960
Page seeded by OCA on Thu Feb 21 11:45:56 2008
Categories: Homo sapiens | Single protein | Seehra, J S. | Somers, W S. | Stahl, M. | SO4 | TLA | Cytokine | Glycoprotein | Interleukin | Receptor | Signaling
