1b9k
From Proteopedia
(New page: 200px<br /><applet load="1b9k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b9k, resolution 1.90Å" /> '''ALPHA-ADAPTIN APPEND...) |
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| - | [[Image:1b9k.gif|left|200px]]<br /><applet load="1b9k" size=" | + | [[Image:1b9k.gif|left|200px]]<br /><applet load="1b9k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1b9k, resolution 1.90Å" /> | caption="1b9k, resolution 1.90Å" /> | ||
'''ALPHA-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2'''<br /> | '''ALPHA-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The alpha subunit of the endocytotic AP2 adaptor complex contains a 30 kDa | + | The alpha subunit of the endocytotic AP2 adaptor complex contains a 30 kDa "appendage" domain, which is joined to the rest of the protein via a flexible linker. The 1.9 A resolution crystal structure of this domain reveals a single binding site for its ligands, which include amphiphysin, Eps15, and epsin. This domain when overexpressed in COS7 fibroblasts is shown to inhibit transferrin uptake, whereas mutants in which interactions with its binding partners are abolished do not. DPF/W motifs present in appendage domain-binding partners are shown to play a crucial role in their interactions with the domain. A single site for binding multiple ligands would allow for temporal and spatial regulation in the recruitment of components of the endocytic machinery. |
==About this Structure== | ==About this Structure== | ||
| - | 1B9K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1B9K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B9K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Evans, P | + | [[Category: Evans, P R.]] |
| - | [[Category: Owen, D | + | [[Category: Owen, D J.]] |
[[Category: adaptor]] | [[Category: adaptor]] | ||
[[Category: endocytosis]] | [[Category: endocytosis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:52:56 2008'' |
Revision as of 09:52, 21 February 2008
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ALPHA-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2
Overview
The alpha subunit of the endocytotic AP2 adaptor complex contains a 30 kDa "appendage" domain, which is joined to the rest of the protein via a flexible linker. The 1.9 A resolution crystal structure of this domain reveals a single binding site for its ligands, which include amphiphysin, Eps15, and epsin. This domain when overexpressed in COS7 fibroblasts is shown to inhibit transferrin uptake, whereas mutants in which interactions with its binding partners are abolished do not. DPF/W motifs present in appendage domain-binding partners are shown to play a crucial role in their interactions with the domain. A single site for binding multiple ligands would allow for temporal and spatial regulation in the recruitment of components of the endocytic machinery.
About this Structure
1B9K is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
A structural explanation for the binding of multiple ligands by the alpha-adaptin appendage domain., Owen DJ, Vallis Y, Noble ME, Hunter JB, Dafforn TR, Evans PR, McMahon HT, Cell. 1999 Jun 11;97(6):805-15. PMID:10380931
Page seeded by OCA on Thu Feb 21 11:52:56 2008
