1ba3
From Proteopedia
(New page: 200px<br /><applet load="1ba3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ba3, resolution 2.2Å" /> '''FIREFLY LUCIFERASE IN...) |
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| - | [[Image:1ba3.gif|left|200px]]<br /><applet load="1ba3" size=" | + | [[Image:1ba3.gif|left|200px]]<br /><applet load="1ba3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ba3, resolution 2.2Å" /> | caption="1ba3, resolution 2.2Å" /> | ||
'''FIREFLY LUCIFERASE IN COMPLEX WITH BROMOFORM'''<br /> | '''FIREFLY LUCIFERASE IN COMPLEX WITH BROMOFORM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The firefly luciferase enzyme from Photinus pyralis is probably the | + | The firefly luciferase enzyme from Photinus pyralis is probably the best-characterized model system for studying anesthetic-protein interactions. It binds a diverse range of general anesthetics over a large potency range, displays a sensitivity to anesthetics that is very similar to that found in animals, and has an anesthetic sensitivity that can be modulated by one of its substrates (ATP). In this paper we describe the properties of bromoform acting as a general anesthetic (in Rana temporaria tadpoles) and as an inhibitor of the firefly luciferase enzyme at high and low ATP concentrations. In addition, we describe the crystal structure of the low-ATP form of the luciferase enzyme in the presence of bromoform at 2.2-A resolution. These results provide a structural basis for understanding the anesthetic inhibition of the enzyme, as well as an explanation for the ATP modulation of its anesthetic sensitivity. |
==About this Structure== | ==About this Structure== | ||
| - | 1BA3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis] with MBR as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] Full crystallographic information is available from [http:// | + | 1BA3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis] with <scene name='pdbligand=MBR:'>MBR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BA3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Brick, P.]] | [[Category: Brick, P.]] | ||
[[Category: Conti, E.]] | [[Category: Conti, E.]] | ||
| - | [[Category: Franks, N | + | [[Category: Franks, N P.]] |
[[Category: Jenkins, A.]] | [[Category: Jenkins, A.]] | ||
| - | [[Category: Lieb, W | + | [[Category: Lieb, W R.]] |
[[Category: MBR]] | [[Category: MBR]] | ||
[[Category: luminescence]] | [[Category: luminescence]] | ||
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[[Category: photoprotein]] | [[Category: photoprotein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:53:11 2008'' |
Revision as of 09:53, 21 February 2008
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FIREFLY LUCIFERASE IN COMPLEX WITH BROMOFORM
Overview
The firefly luciferase enzyme from Photinus pyralis is probably the best-characterized model system for studying anesthetic-protein interactions. It binds a diverse range of general anesthetics over a large potency range, displays a sensitivity to anesthetics that is very similar to that found in animals, and has an anesthetic sensitivity that can be modulated by one of its substrates (ATP). In this paper we describe the properties of bromoform acting as a general anesthetic (in Rana temporaria tadpoles) and as an inhibitor of the firefly luciferase enzyme at high and low ATP concentrations. In addition, we describe the crystal structure of the low-ATP form of the luciferase enzyme in the presence of bromoform at 2.2-A resolution. These results provide a structural basis for understanding the anesthetic inhibition of the enzyme, as well as an explanation for the ATP modulation of its anesthetic sensitivity.
About this Structure
1BA3 is a Single protein structure of sequence from Photinus pyralis with as ligand. Active as Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing), with EC number 1.13.12.7 Full crystallographic information is available from OCA.
Reference
Structural basis for the inhibition of firefly luciferase by a general anesthetic., Franks NP, Jenkins A, Conti E, Lieb WR, Brick P, Biophys J. 1998 Nov;75(5):2205-11. PMID:9788915
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