1bvs
From Proteopedia
(New page: 200px<br /><applet load="1bvs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bvs, resolution 3.00Å" /> '''RUVA COMPLEXED TO A ...) |
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| - | [[Image:1bvs.gif|left|200px]]<br /><applet load="1bvs" size=" | + | [[Image:1bvs.gif|left|200px]]<br /><applet load="1bvs" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bvs, resolution 3.00Å" /> | caption="1bvs, resolution 3.00Å" /> | ||
'''RUVA COMPLEXED TO A HOLLIDAY JUNCTION.'''<br /> | '''RUVA COMPLEXED TO A HOLLIDAY JUNCTION.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Holliday junctions occur as intermediates in homologous recombination and | + | Holliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease RuvC, which nicks two strands. The crystal structure of a complex between the RuvA protein of M. leprae and a synthetic four-way junction has now been determined. Rather than binding on the open surface of a RuvA tetramer as previously suggested, the DNA is sandwiched between two RuvA tetramers, which form a closed octameric shell, stabilized by a conserved tetramer-tetramer interface. Interactions between the DNA backbone and helix-hairpin-helix motifs from both tetramers suggest a mechanism for strand separation promoted by RuvA. |
==About this Structure== | ==About this Structure== | ||
| - | 1BVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http:// | + | 1BVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mycobacterium leprae]] | [[Category: Mycobacterium leprae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Pearl, L | + | [[Category: Pearl, L H.]] |
| - | [[Category: Roe, S | + | [[Category: Roe, S M.]] |
[[Category: branch migration]] | [[Category: branch migration]] | ||
[[Category: dna repair]] | [[Category: dna repair]] | ||
[[Category: holliday junction resolvase component]] | [[Category: holliday junction resolvase component]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:59:37 2008'' |
Revision as of 09:59, 21 February 2008
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RUVA COMPLEXED TO A HOLLIDAY JUNCTION.
Overview
Holliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease RuvC, which nicks two strands. The crystal structure of a complex between the RuvA protein of M. leprae and a synthetic four-way junction has now been determined. Rather than binding on the open surface of a RuvA tetramer as previously suggested, the DNA is sandwiched between two RuvA tetramers, which form a closed octameric shell, stabilized by a conserved tetramer-tetramer interface. Interactions between the DNA backbone and helix-hairpin-helix motifs from both tetramers suggest a mechanism for strand separation promoted by RuvA.
About this Structure
1BVS is a Single protein structure of sequence from Mycobacterium leprae. Full crystallographic information is available from OCA.
Reference
Crystal structure of an octameric RuvA-Holliday junction complex., Roe SM, Barlow T, Brown T, Oram M, Keeley A, Tsaneva IR, Pearl LH, Mol Cell. 1998 Sep;2(3):361-72. PMID:9774974
Page seeded by OCA on Thu Feb 21 11:59:37 2008
