1c8c
From Proteopedia
(New page: 200px<br /><applet load="1c8c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c8c, resolution 1.45Å" /> '''CRYSTAL STRUCTURES O...) |
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| - | [[Image:1c8c.gif|left|200px]]<br /><applet load="1c8c" size=" | + | [[Image:1c8c.gif|left|200px]]<br /><applet load="1c8c" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1c8c, resolution 1.45Å" /> | caption="1c8c, resolution 1.45Å" /> | ||
'''CRYSTAL STRUCTURES OF THE CHROMOSOMAL PROTEINS SSO7D/SAC7D BOUND TO DNA CONTAINING T-G MISMATCHED BASE PAIRS'''<br /> | '''CRYSTAL STRUCTURES OF THE CHROMOSOMAL PROTEINS SSO7D/SAC7D BOUND TO DNA CONTAINING T-G MISMATCHED BASE PAIRS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Sso7d and Sac7d are two small chromatin proteins from the | + | Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation. |
==About this Structure== | ==About this Structure== | ||
| - | 1C8C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http:// | + | 1C8C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus solfataricus]] | [[Category: Sulfolobus solfataricus]] | ||
| - | [[Category: Edmondson, S | + | [[Category: Edmondson, S P.]] |
| - | [[Category: Gao, Y | + | [[Category: Gao, Y G.]] |
| - | [[Category: Liaw, Y | + | [[Category: Liaw, Y C.]] |
[[Category: Robinson, H.]] | [[Category: Robinson, H.]] | ||
| - | [[Category: Shriver, J | + | [[Category: Shriver, J W.]] |
[[Category: Su, S.]] | [[Category: Su, S.]] | ||
| - | [[Category: Wang, A | + | [[Category: Wang, A H.J.]] |
[[Category: achaeabacteria]] | [[Category: achaeabacteria]] | ||
[[Category: dna binding protein]] | [[Category: dna binding protein]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:03:26 2008'' |
Revision as of 10:03, 21 February 2008
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CRYSTAL STRUCTURES OF THE CHROMOSOMAL PROTEINS SSO7D/SAC7D BOUND TO DNA CONTAINING T-G MISMATCHED BASE PAIRS
Overview
Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation.
About this Structure
1C8C is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs., Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH, J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:11031116
Page seeded by OCA on Thu Feb 21 12:03:26 2008
Categories: Single protein | Sulfolobus solfataricus | Edmondson, S P. | Gao, Y G. | Liaw, Y C. | Robinson, H. | Shriver, J W. | Su, S. | Wang, A H.J. | Achaeabacteria | Dna binding protein | Electrostatics | Hyperthermophile | Molecular modeling | Protein stability | Protein-dna interaction | T-g mismatch | X-ray crystallography
