1cfl
From Proteopedia
(New page: 200px<br /><applet load="1cfl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cfl" /> '''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADD...) |
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| - | [[Image:1cfl.gif|left|200px]]<br /><applet load="1cfl" size=" | + | [[Image:1cfl.gif|left|200px]]<br /><applet load="1cfl" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT'''<br /> | '''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct] is one of the | + | The pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct] is one of the major photoproducts induced by UV irradiation of DNA and occurs at TpT sites. The (6-4) adduct is highly mutagenic and leads most often to a 3' T --> C transition with 85% replicating error frequency [LeClerc, J. E., Borden, A. & Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88, 9685-9689]. To determine the origin of the specific 3' T --> C transition of the (6-4) adduct, we have used experimental NMR restraints and molecular dynamics to determine the solution structure of a (6-4)-lesion DNA decamer duplex that contains a mismatched base pair between the 3' T residue and an opposed G residue. Normal Watson-Crick-type hydrogen bonding is retained at the 5' T of the lesion site. The O2 carbonyl of the 3' T residue forms hydrogen bonds with the imino and amino protons of the opposed G residue. This potential hydrogen bonding stabilizes the overall helix and restores the highly distorted conformation of the (6-4) adduct to the typical B-form-like DNA structure. This structural feature can explain the marked preference for the insertion of an A residue opposite the 5' T and a G residue opposite the 3' T of the (6-4) lesion during trans-lesion synthesis. Thus these insertions yield the predominant 3' T --> C transition. |
==About this Structure== | ==About this Structure== | ||
| - | 1CFL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1CFL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFL OCA]. |
==Reference== | ==Reference== | ||
Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct., Lee JH, Hwang GS, Choi BS, Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6632-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10359763 10359763] | Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct., Lee JH, Hwang GS, Choi BS, Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6632-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10359763 10359763] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Choi, B | + | [[Category: Choi, B S.]] |
| - | [[Category: Hwang, G | + | [[Category: Hwang, G S.]] |
| - | [[Category: Lee, J | + | [[Category: Lee, J H.]] |
[[Category: (6-4) adduct]] | [[Category: (6-4) adduct]] | ||
[[Category: deoxyribonucleic acid]] | [[Category: deoxyribonucleic acid]] | ||
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[[Category: nmr]] | [[Category: nmr]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:35 2008'' |
Revision as of 10:05, 21 February 2008
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DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT
Overview
The pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct] is one of the major photoproducts induced by UV irradiation of DNA and occurs at TpT sites. The (6-4) adduct is highly mutagenic and leads most often to a 3' T --> C transition with 85% replicating error frequency [LeClerc, J. E., Borden, A. & Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88, 9685-9689]. To determine the origin of the specific 3' T --> C transition of the (6-4) adduct, we have used experimental NMR restraints and molecular dynamics to determine the solution structure of a (6-4)-lesion DNA decamer duplex that contains a mismatched base pair between the 3' T residue and an opposed G residue. Normal Watson-Crick-type hydrogen bonding is retained at the 5' T of the lesion site. The O2 carbonyl of the 3' T residue forms hydrogen bonds with the imino and amino protons of the opposed G residue. This potential hydrogen bonding stabilizes the overall helix and restores the highly distorted conformation of the (6-4) adduct to the typical B-form-like DNA structure. This structural feature can explain the marked preference for the insertion of an A residue opposite the 5' T and a G residue opposite the 3' T of the (6-4) lesion during trans-lesion synthesis. Thus these insertions yield the predominant 3' T --> C transition.
About this Structure
1CFL is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct., Lee JH, Hwang GS, Choi BS, Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6632-6. PMID:10359763
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