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3daa
From Proteopedia
(New page: 200px<br /> <applet load="3daa" size="450" color="white" frame="true" align="right" spinBox="true" caption="3daa, resolution 1.9Å" /> '''CRYSTALLOGRAPHIC STR...) |
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==About this Structure== | ==About this Structure== | ||
| - | 3DAA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]] with PDD as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.21 2.6.1.21]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3DAA OCA]]. | + | 3DAA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]] with PDD as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/D-amino-acid_transaminase D-amino-acid transaminase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.21 2.6.1.21]]. Structure known Active Sites: ASA and ASB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3DAA OCA]]. |
==Reference== | ==Reference== | ||
Crystallographic study of steps along the reaction pathway of D-amino acid aminotransferase., Peisach D, Chipman DM, Van Ophem PW, Manning JM, Ringe D, Biochemistry. 1998 Apr 7;37(14):4958-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9538014 9538014] | Crystallographic study of steps along the reaction pathway of D-amino acid aminotransferase., Peisach D, Chipman DM, Van Ophem PW, Manning JM, Ringe D, Biochemistry. 1998 Apr 7;37(14):4958-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9538014 9538014] | ||
[[Category: Bacillus sp.]] | [[Category: Bacillus sp.]] | ||
| + | [[Category: D-amino-acid transaminase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chipman, D.M.]] | [[Category: Chipman, D.M.]] | ||
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[[Category: transaminase]] | [[Category: transaminase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:46:59 2007'' |
Revision as of 11:42, 30 October 2007
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CRYSTALLOGRAPHIC STRUCTURE OF D-AMINO ACID AMINOTRANSFERASE INACTIVATED BY PYRIDOXYL-D-ALANINE
Overview
The three-dimensional structures of two forms of the D-amino acid, aminotransferase (D-aAT) from Bacillus sp. YM-1 have been determined, crystallographically: the pyridoxal phosphate (PLP) form and a complex, with the reduced analogue of the external aldimine, N-(5'-phosphopyridoxyl)-d-alanine (PPDA). Together with the previously, reported pyridoxamine phosphate form of the enzyme [Sugio et al. (1995), Biochemistry 34, 9661], these structures allow us to describe the pathway, of the enzymatic reaction in structural terms. A major determinant of the, enzyme's stereospecificity for D-amino acids is a group of three residues, (Tyr30, Arg98, and His100, with the latter two contributed by the, neighboring subunit) forming four hydrogen bonds to the substrate, alpha-carboxyl group. The ... [(full description)]
About this Structure
3DAA is a [Single protein] structure of sequence from [Bacillus sp.] with PDD as [ligand]. Active as [D-amino-acid transaminase], with EC number [2.6.1.21]. Structure known Active Sites: ASA and ASB. Full crystallographic information is available from [OCA].
Reference
Crystallographic study of steps along the reaction pathway of D-amino acid aminotransferase., Peisach D, Chipman DM, Van Ophem PW, Manning JM, Ringe D, Biochemistry. 1998 Apr 7;37(14):4958-67. PMID:9538014
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