1du6
From Proteopedia
(New page: 200px<br /><applet load="1du6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1du6" /> '''SOLUTION STRUCTURE OF THE TRUNCATED PBX HOME...) |
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'''SOLUTION STRUCTURE OF THE TRUNCATED PBX HOMEODOMAIN'''<br /> | '''SOLUTION STRUCTURE OF THE TRUNCATED PBX HOMEODOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | PBX is a member of the three amino acid loop extension (TALE) class of | + | PBX is a member of the three amino acid loop extension (TALE) class of homeodomains. PBX binds DNA cooperatively with HOX homeodomain proteins that contain a conserved YPWM motif. The amino acids immediately C-terminal to the PBX homeodomain increase the affinity of the homeodomain for its DNA site and HOX proteins. We have determined the structure of the free PBX homeodomain using NMR spectroscopy. Both the PBX homeodomain and the extended PBX homeodomain make identical contacts with a 5'-TGAT-3' DNA site and a YPWM peptide. A fourth alpha-helix, which forms upon binding to DNA, stabilizes the extended PBX structure. Variations in DNA sequence selectivity of heterodimeric PBX-HOX complexes depend on the HOX partner; however, a comparison of five different HOX-derived YPWM peptides showed that each bound to PBX in the same way, differing only in the strength of the association. |
==About this Structure== | ==About this Structure== | ||
| - | 1DU6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1DU6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DU6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: homeodomain]] | [[Category: homeodomain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:20:31 2008'' |
Revision as of 10:20, 21 February 2008
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SOLUTION STRUCTURE OF THE TRUNCATED PBX HOMEODOMAIN
Overview
PBX is a member of the three amino acid loop extension (TALE) class of homeodomains. PBX binds DNA cooperatively with HOX homeodomain proteins that contain a conserved YPWM motif. The amino acids immediately C-terminal to the PBX homeodomain increase the affinity of the homeodomain for its DNA site and HOX proteins. We have determined the structure of the free PBX homeodomain using NMR spectroscopy. Both the PBX homeodomain and the extended PBX homeodomain make identical contacts with a 5'-TGAT-3' DNA site and a YPWM peptide. A fourth alpha-helix, which forms upon binding to DNA, stabilizes the extended PBX structure. Variations in DNA sequence selectivity of heterodimeric PBX-HOX complexes depend on the HOX partner; however, a comparison of five different HOX-derived YPWM peptides showed that each bound to PBX in the same way, differing only in the strength of the association.
About this Structure
1DU6 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Conformational changes in the PBX homeodomain and C-terminal extension upon binding DNA and HOX-derived YPWM peptides., Sprules T, Green N, Featherstone M, Gehring K, Biochemistry. 2000 Aug 15;39(32):9943-50. PMID:10933814
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