1e4e
From Proteopedia
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==Overview== | ==Overview== | ||
| - | d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly | + | d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows that the active site has unexpected interactions and is distinct from previous models for d-alanyl-d-lactate ligase mechanistic studies. It appears that the preference of the enzyme for lactate as a ligand over d-alanine could be mediated by electrostatic effects and/or a hydrogen-bonding network, which principally involve His-244. The structure of d-alanyl-d-lactate ligase provides a revised interpretation of the molecular events that lead to vancomycin resistance. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Enterococcus faecium]] | [[Category: Enterococcus faecium]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Roper, D | + | [[Category: Roper, D I.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: plasmid]] | [[Category: plasmid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:23:44 2008'' |
Revision as of 10:23, 21 February 2008
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D-ALANYL-D-LACATE LIGASE
Overview
d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows that the active site has unexpected interactions and is distinct from previous models for d-alanyl-d-lactate ligase mechanistic studies. It appears that the preference of the enzyme for lactate as a ligand over d-alanine could be mediated by electrostatic effects and/or a hydrogen-bonding network, which principally involve His-244. The structure of d-alanyl-d-lactate ligase provides a revised interpretation of the molecular events that lead to vancomycin resistance.
About this Structure
1E4E is a Protein complex structure of sequences from Enterococcus faecium with , , , and as ligands. Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
Reference
The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA)., Roper DI, Huyton T, Vagin A, Dodson G, Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8921-5. PMID:10908650
Page seeded by OCA on Thu Feb 21 12:23:44 2008
Categories: Enterococcus faecium | Protein complex | Roper, D I. | ADP | GOL | MG | PHY | SO4 | Antibiotic resistance | Cell wall | Ligase | Membrane | Peptidoglycan synthesis | Plasmid
