1eo8
From Proteopedia
(New page: 200px<br /> <applet load="1eo8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eo8, resolution 2.8Å" /> '''INFLUENZA VIRUS HEMA...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1eo8.gif|left|200px]]<br /> | + | [[Image:1eo8.gif|left|200px]]<br /><applet load="1eo8" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1eo8" size=" | + | |
caption="1eo8, resolution 2.8Å" /> | caption="1eo8, resolution 2.8Å" /> | ||
'''INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH A NEUTRALIZING ANTIBODY'''<br /> | '''INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH A NEUTRALIZING ANTIBODY'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of a complex between the hemagglutinin of influenza virus | + | The structure of a complex between the hemagglutinin of influenza virus and the Fab of a neutralizing antibody was determined by X-ray crystallography at 2.8 A resolution. This antibody and another which has only 56% sequence identity bind to the same epitope with very similar affinities and in the same orientation. One third of the interactions is conserved in the two complexes; a significant proportion of the interactions that differ are established by residues of the H3 complementarity-determining regions (CDR) which adopt distinct conformations in the two antibodies. This demonstrates that there is a definite flexibility in the selection of antibodies that bind to a given epitope, despite the high affinity of their complexes. This flexibility allows the humoral immune response to be redundant, a feature that may be useful in achieving longer lasting protection against evolving viral pathogens. |
==About this Structure== | ==About this Structure== | ||
| - | 1EO8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1EO8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EO8 OCA]. |
==Reference== | ==Reference== | ||
| Line 16: | Line 15: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bizebard, T.]] | [[Category: Bizebard, T.]] | ||
| - | [[Category: Daniels, R | + | [[Category: Daniels, R S.]] |
[[Category: Fleury, D.]] | [[Category: Fleury, D.]] | ||
[[Category: Gigant, B.]] | [[Category: Gigant, B.]] | ||
[[Category: Knossow, M.]] | [[Category: Knossow, M.]] | ||
| - | [[Category: Skehel, J | + | [[Category: Skehel, J J.]] |
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: complex (hemagglutinin/immmunoglobulin)]] | [[Category: complex (hemagglutinin/immmunoglobulin)]] | ||
| Line 28: | Line 27: | ||
[[Category: viral protein]] | [[Category: viral protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:29:49 2008'' |
Revision as of 10:29, 21 February 2008
|
INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH A NEUTRALIZING ANTIBODY
Overview
The structure of a complex between the hemagglutinin of influenza virus and the Fab of a neutralizing antibody was determined by X-ray crystallography at 2.8 A resolution. This antibody and another which has only 56% sequence identity bind to the same epitope with very similar affinities and in the same orientation. One third of the interactions is conserved in the two complexes; a significant proportion of the interactions that differ are established by residues of the H3 complementarity-determining regions (CDR) which adopt distinct conformations in the two antibodies. This demonstrates that there is a definite flexibility in the selection of antibodies that bind to a given epitope, despite the high affinity of their complexes. This flexibility allows the humoral immune response to be redundant, a feature that may be useful in achieving longer lasting protection against evolving viral pathogens.
About this Structure
1EO8 is a Protein complex structure of sequences from Influenza a virus and Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Structural evidence for recognition of a single epitope by two distinct antibodies., Fleury D, Daniels RS, Skehel JJ, Knossow M, Bizebard T, Proteins. 2000 Sep 1;40(4):572-8. PMID:10899782
Page seeded by OCA on Thu Feb 21 12:29:49 2008
