1evo
From Proteopedia
(New page: 200px<br /><applet load="1evo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1evo" /> '''NMR OBSERVATION OF A NOVEL C-TETRAD'''<br />...) |
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'''NMR OBSERVATION OF A NOVEL C-TETRAD'''<br /> | '''NMR OBSERVATION OF A NOVEL C-TETRAD'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report the NMR structure of the DNA sequence d-TGGGCGGT in Na(+) | + | We report the NMR structure of the DNA sequence d-TGGGCGGT in Na(+) solutions at neutral pH, containing a repeat sequence from SV40 viral genome. The structure is a novel quadruplex incorporating the C-tetrad formed by symmetrical pairing of four Cs via NH(2)&bond;O(2) H-bonds in a plane. The C-tetrad has a wider cavity compared to G-tetrads and stacks well over the adjacent G4-tetrad, but poorly on the G6 tetrad. The quadruplex helix is largely underwound by 8-10 degrees compared to B-DNA except at the C5-G6 step. To our knowledge this is the first report of C-tetrad formation in DNA structures, and would be of significance from the point of view of both structural diversity and specific recognition. |
==About this Structure== | ==About this Structure== | ||
| - | 1EVO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1EVO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVO OCA]. |
==Reference== | ==Reference== | ||
NMR observation of a novel C-tetrad in the structure of the SV40 repeat sequence GGGCGG., Patel PK, Bhavesh NS, Hosur RV, Biochem Biophys Res Commun. 2000 Apr 21;270(3):967-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10772934 10772934] | NMR observation of a novel C-tetrad in the structure of the SV40 repeat sequence GGGCGG., Patel PK, Bhavesh NS, Hosur RV, Biochem Biophys Res Commun. 2000 Apr 21;270(3):967-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10772934 10772934] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bhavesh, N | + | [[Category: Bhavesh, N S.]] |
| - | [[Category: Hosur, R | + | [[Category: Hosur, R V.]] |
| - | [[Category: Patel, P | + | [[Category: Patel, P K.]] |
[[Category: nmr structure; g-quadruplex; c-tetrad; gggcgg repeat]] | [[Category: nmr structure; g-quadruplex; c-tetrad; gggcgg repeat]] | ||
[[Category: sv40]] | [[Category: sv40]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:32:02 2008'' |
Revision as of 10:32, 21 February 2008
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NMR OBSERVATION OF A NOVEL C-TETRAD
Overview
We report the NMR structure of the DNA sequence d-TGGGCGGT in Na(+) solutions at neutral pH, containing a repeat sequence from SV40 viral genome. The structure is a novel quadruplex incorporating the C-tetrad formed by symmetrical pairing of four Cs via NH(2)&bond;O(2) H-bonds in a plane. The C-tetrad has a wider cavity compared to G-tetrads and stacks well over the adjacent G4-tetrad, but poorly on the G6 tetrad. The quadruplex helix is largely underwound by 8-10 degrees compared to B-DNA except at the C5-G6 step. To our knowledge this is the first report of C-tetrad formation in DNA structures, and would be of significance from the point of view of both structural diversity and specific recognition.
About this Structure
1EVO is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR observation of a novel C-tetrad in the structure of the SV40 repeat sequence GGGCGG., Patel PK, Bhavesh NS, Hosur RV, Biochem Biophys Res Commun. 2000 Apr 21;270(3):967-71. PMID:10772934
Page seeded by OCA on Thu Feb 21 12:32:02 2008
